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Sample GSM1087634 Query DataSets for GSM1087634
Status Public on Apr 01, 2013
Title QGY, biological rep3
Sample type RNA
Source name control clone
Organism Homo sapiens
Characteristics cell line: QGY-7703
cell type: human hepatocellular carcinoma
Treatment protocol No treatment was done.
Growth protocol Steady-state proliferating cells.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed using QIAGEN columns according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250 ng total RNA (GeneChip 3' IVT Express Kit, P/N 702646 Rev. 8, Affymetrix).
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133A 2.0 Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using protocol FS450_0002.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000 7G.
Description QGY_3
Gene expression data from control clone.
Data processing The data were analyzed with Affymetrix Expression Console software using Affymetrix default analysis settings and RMA as the normalization method.
Submission date Feb 23, 2013
Last update date Apr 01, 2013
Contact name Myrna Serrano
Organization name Virginia Commonwealth University
Street address 1101 East Marshall St
City Richmond
State/province VA
ZIP/Postal code 23298
Country USA
Platform ID GPL571
Series (1)
GSE44601 Expression data from SND1 knockdown clones of human HCC cell line QGY-7703

Data table header descriptions
VALUE Log2 RMA signal

Data table
1007_s_at 9.813513
1053_at 8.812672
117_at 4.810751
121_at 7.442204
1255_g_at 2.809177
1294_at 5.027735
1316_at 6.07302
1320_at 4.153179
1405_i_at 3.468626
1431_at 3.713526
1438_at 5.243263
1487_at 8.562313
1494_f_at 5.322575
1598_g_at 7.751542
160020_at 6.055274
1729_at 7.55514
177_at 5.388894
1773_at 5.55455
179_at 7.817871
1861_at 6.930053

Total number of rows: 22277

Table truncated, full table size 431 Kbytes.

Supplementary file Size Download File type/resource
GSM1087634_Qgy-2-Rep3_HG-U133A_2_.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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