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Status |
Public on Oct 01, 2013 |
Title |
AEC_92_male_21O2_VitDctrl |
Sample type |
RNA |
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Source name |
Isolated and in vitro cultured placental cells, arterial endothelial cells, male
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Organism |
Homo sapiens |
Characteristics |
tissue: placenta cell type: arterial endothelial cells gender: male
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Treatment protocol |
No treatments.
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Growth protocol |
Prior to microarray analysis, 800,000 cells were seeded on 1% (v/v) gelatin-coated flasks (75 cm²) using Endothelial Basal Medium (EBM, Cambrex, Clonetics, USA) supplemented with the EGM-MV BulletKit (Clonetics). Endothelial cells were cultured for 48 h at 21% O2/5% CO2 environment at 37°C.
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Extracted molecule |
total RNA |
Extraction protocol |
RNA isolation with the Qiagen Mini kit according to the manufacturer.
|
Label |
biotin
|
Label protocol |
The Ambion® WT Expression Kit; For Affymetrix® GeneChip® Whole Transcript (WT) Expression Arrays, 09/2009 Rev C (Cat No. 4411973 ), Affymetrix WT Terminal Labeling Kit 30rxn (Cat No. 900671).
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Hybridization protocol |
GeneChip® HT hybridization, Wash and Stain Kit (Cat No. 900720); Affymetrix GeneChip® Fluidics Station 450: protocol FS450_0007 for Cartridge Arrays.
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Scan protocol |
Affymetrix Scanner: GCS3000; AGCC (Command Console Software AGCC_3_1_1).
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Description |
male_ECA_92_5_b_9_HuGene-1_0-st-v1
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Data processing |
Gene Expression Console v.1.1, Affymetrix (Expression Console EC_1_1_2) using Affymetrix default analysis settings for generation of CEL files. Data Pre-Processing (Partek Software, v.6.5: RMA (background correction, quantile normalization across all chips in the experiment, log2 transformation, median polish summarization)); Biostatistical data analysis: R/Bioconductor.
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Submission date |
Feb 17, 2013 |
Last update date |
Oct 01, 2013 |
Contact name |
Silvija Tokic |
E-mail(s) |
silvija.tokic@medunigraz.at
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Organization name |
Medical University of Graz
|
Street address |
Auenbruggerplatz 14
|
City |
Graz |
ZIP/Postal code |
8036 |
Country |
Austria |
|
|
Platform ID |
GPL6244 |
Series (1) |
GSE44368 |
The human placental sexome differs between trophoblast epithelium and villous vessel endothelium |
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