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Sample GSM1084000 Query DataSets for GSM1084000
Status Public on Oct 01, 2013
Title AEC_92_male_21O2_VitDctrl
Sample type RNA
 
Source name Isolated and in vitro cultured placental cells, arterial endothelial cells, male
Organism Homo sapiens
Characteristics tissue: placenta
cell type: arterial endothelial cells
gender: male
Treatment protocol No treatments.
Growth protocol Prior to microarray analysis, 800,000 cells were seeded on 1% (v/v) gelatin-coated flasks (75 cm²) using Endothelial Basal Medium (EBM, Cambrex, Clonetics, USA) supplemented with the EGM-MV BulletKit (Clonetics). Endothelial cells were cultured for 48 h at 21% O2/5% CO2 environment at 37°C.
Extracted molecule total RNA
Extraction protocol RNA isolation with the Qiagen Mini kit according to the manufacturer.
Label biotin
Label protocol The Ambion® WT Expression Kit; For Affymetrix® GeneChip® Whole Transcript (WT) Expression Arrays, 09/2009 Rev C (Cat No. 4411973 ), Affymetrix WT Terminal Labeling Kit 30rxn (Cat No. 900671).
 
Hybridization protocol GeneChip® HT hybridization, Wash and Stain Kit (Cat No. 900720); Affymetrix GeneChip® Fluidics Station 450: protocol FS450_0007 for Cartridge Arrays.
Scan protocol Affymetrix Scanner: GCS3000; AGCC (Command Console Software AGCC_3_1_1).
Description male_ECA_92_5_b_9_HuGene-1_0-st-v1
Data processing Gene Expression Console v.1.1, Affymetrix (Expression Console EC_1_1_2) using Affymetrix default analysis settings for generation of CEL files.
Data Pre-Processing (Partek Software, v.6.5: RMA (background correction, quantile normalization across all chips in the experiment, log2 transformation, median polish summarization)); Biostatistical data analysis: R/Bioconductor.
 
Submission date Feb 17, 2013
Last update date Oct 01, 2013
Contact name Silvija Tokic
E-mail(s) silvija.tokic@medunigraz.at
Organization name Medical University of Graz
Street address Auenbruggerplatz 14
City Graz
ZIP/Postal code 8036
Country Austria
 
Platform ID GPL6244
Series (1)
GSE44368 The human placental sexome differs between trophoblast epithelium and villous vessel endothelium

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
8176719 9.90206
8176624 9.62083
8176375 10.6085
8177232 7.89563
8176578 6.82062
8176384 7.34289
8177137 7.57279
8176730 5.83301
8176698 6.36605
8166500 8.6304
8172827 9.10229
8171747 10.3235
8176460 7.84315
7912852 11.1318
8166876 10.7486
8171222 9.92043
8176709 5.14664
8172876 8.55313
8177217 7.81686
8166956 8.37983

Total number of rows: 33297

Table truncated, full table size 516 Kbytes.




Supplementary file Size Download File type/resource
GSM1084000_male_ECA_92_5_b_9_HuGene-1_0-st-v1_.CEL.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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