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Sample GSM1083993 Query DataSets for GSM1083993
Status Public on Oct 01, 2013
Title AEC_84f_female_21O2_VitDctrl
Sample type RNA
 
Source name Isolated and in vitro cultured placental cells, arterial endothelial cells, female
Organism Homo sapiens
Characteristics tissue: placenta
cell type: arterial endothelial cells
gender: female
Treatment protocol No treatments.
Growth protocol Prior to microarray analysis, 800,000 cells were seeded on 1% (v/v) gelatin-coated flasks (75 cm²) using Endothelial Basal Medium (EBM, Cambrex, Clonetics, USA) supplemented with the EGM-MV BulletKit (Clonetics). Endothelial cells were cultured for 48 h at 21% O2/5% CO2 environment at 37°C.
Extracted molecule total RNA
Extraction protocol RNA isolation with the Qiagen Mini kit according to the manufacturer.
Label biotin
Label protocol The Ambion® WT Expression Kit; For Affymetrix® GeneChip® Whole Transcript (WT) Expression Arrays, 09/2009 Rev C (Cat No. 4411973 ), Affymetrix WT Terminal Labeling Kit 30rxn (Cat No. 900671).
 
Hybridization protocol GeneChip® HT hybridization, Wash and Stain Kit (Cat No. 900720); Affymetrix GeneChip® Fluidics Station 450: protocol FS450_0007 for Cartridge Arrays.
Scan protocol Affymetrix Scanner: GCS3000; AGCC (Command Console Software AGCC_3_1_1).
Description female_ECA_84_7_b_1_HuGene-1_0-st-v1
Data processing Gene Expression Console v.1.1, Affymetrix (Expression Console EC_1_1_2) using Affymetrix default analysis settings for generation of CEL files.
Data Pre-Processing (Partek Software, v.6.5: RMA (background correction, quantile normalization across all chips in the experiment, log2 transformation, median polish summarization)); Biostatistical data analysis: R/Bioconductor.
 
Submission date Feb 17, 2013
Last update date Oct 01, 2013
Contact name Silvija Tokic
E-mail(s) silvija.tokic@medunigraz.at
Organization name Medical University of Graz
Street address Auenbruggerplatz 14
City Graz
ZIP/Postal code 8036
Country Austria
 
Platform ID GPL6244
Series (1)
GSE44368 The human placental sexome differs between trophoblast epithelium and villous vessel endothelium

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
8176719 6.0404
8176624 5.3452
8176375 5.85538
8177232 6.0785
8176578 5.30669
8176384 5.4223
8177137 5.57894
8176730 5.0948
8176698 4.51343
8166500 9.16844
8172827 9.52826
8171747 10.7778
8176460 7.10765
7912852 11.5726
8166876 10.9506
8171222 10.3678
8176709 4.45727
8172876 8.98567
8177217 7.25683
8166956 8.74468

Total number of rows: 33297

Table truncated, full table size 516 Kbytes.




Supplementary file Size Download File type/resource
GSM1083993_female_ECA_84_7_b_1_HuGene-1_0-st-v1_.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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