|
| Status |
Public on Oct 01, 2013 |
| Title |
SCT_81_male_20O2_VitDctrl |
| Sample type |
RNA |
| |
|
| Source name |
Isolated and in vitro cultured placental cells, syncytiotrophoblasts, male
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: placenta
cell type: syncytiotrophoblasts
gender: male
|
| Treatment protocol |
No treatments.
|
| Growth protocol |
Prior to microarray analysis, 800,000 cells were seeded on 1% (v/v) gelatin-coated flasks (75 cm²) using Endothelial Basal Medium (EBM, Cambrex, Clonetics, USA) supplemented with the EGM-MV BulletKit (Clonetics). Endothelial cells were cultured for 48 h at 21% O2/5% CO2 environment at 37°C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA isolation with the Qiagen Mini kit according to the manufacturer.
|
| Label |
biotin
|
| Label protocol |
The Ambion® WT Expression Kit; For Affymetrix® GeneChip® Whole Transcript (WT) Expression Arrays, 09/2009 Rev C (Cat No. 4411973 ), Affymetrix WT Terminal Labeling Kit 30rxn (Cat No. 900671).
|
| |
|
| Hybridization protocol |
GeneChip® HT hybridization, Wash and Stain Kit (Cat No. 900720); Affymetrix GeneChip® Fluidics Station 450: protocol FS450_0007 for Cartridge Arrays.
|
| Scan protocol |
Affymetrix Scanner: GCS3000; AGCC (Command Console Software AGCC_3_1_1).
|
| Description |
male-S81-C_11_HuGene-1_0-st-v1
|
| Data processing |
Gene Expression Console v.1.1, Affymetrix (Expression Console EC_1_1_2) using Affymetrix default analysis settings for generation of CEL files.
Data Pre-Processing (Partek Software, v.6.5: RMA (background correction, quantile normalization across all chips in the experiment, log2 transformation, median polish summarization)); Biostatistical data analysis: R/Bioconductor.
|
| |
|
| Submission date |
Feb 17, 2013 |
| Last update date |
Oct 01, 2013 |
| Contact name |
Silvija Tokic |
| E-mail(s) |
silvija.tokic@medunigraz.at
|
| Organization name |
Medical University of Graz
|
| Street address |
Auenbruggerplatz 14
|
| City |
Graz |
| ZIP/Postal code |
8036 |
| Country |
Austria |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE44368 |
The human placental sexome differs between trophoblast epithelium and villous vessel endothelium |
|
