GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM1068573 Query DataSets for GSM1068573
Status Public on Jan 20, 2015
Title 99B-928
Sample type RNA
Source name Pat1
Organism Homo sapiens
Characteristics tissue: skeletal muscle
gender: female
age: 14 years
sample type: Congenital Muscular Dystrophy
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from skeletal muscle biopsies with Qiagen (Qiagen, Hilden, Germany) RNeasy Fibrous Tissue mini kit following the manufacturer’s instructions
Label cy3
Label protocol RNA (500 ng) was labeled using Agilent's Low Input RNA Labeling Kit, which involves reverse transcribing the mRNA in the presence of T7-oligo-dT primer to produce cDNA and then in vitro transcribing with T7 RNA polymerase in the presence of Cy3-CTP
Hybridization protocol Labelled cRNAs were hybridised to the Agilent Human SurePrintGE, 8x60K according to manufacturer´s instructions
Scan protocol The arrays were washed, dried by centrifugation and scanned on an Agilent G2565BA microarray scanner at 100% PMT and 5 μm resolution
Description patient reference in corresponding publication: CMD3
Data processing Bioconductor limma package, using background correction (normexp) and quantiles normalization between arrays for final values
Submission date Jan 23, 2013
Last update date Jan 20, 2015
Contact name Susana Kalko
Organization name IDIBAPS
Department Bioinformatics Platform
Street address c/Rosello 153
City Barcelona
State/province BARCELONA
ZIP/Postal code 08036
Country Spain
Platform ID GPL13607
Series (1)
GSE43698 Muscle from patients with mitochondrial myopathies and congenital muscular dystrophies versus normal human muscle

Data table header descriptions
VALUE quantile normalized

Data table
4 8.055028374
5 6.573269454
6 6.475343938
7 10.87448229
8 8.153521777
9 6.218701063
10 6.295631178
11 6.283328643
12 9.523825863
13 9.815600256
14 7.290456903
15 7.525078984
16 6.117744366
17 6.771198153
18 6.077177111
19 6.826674731
20 9.577077043
21 9.000500487
22 6.505199355
23 6.104035464

Total number of rows: 62973

Table truncated, full table size 1089 Kbytes.

Supplementary file Size Download File type/resource
GSM1068573_21_MA_2400252800413961_Cy3_99B-928_2_4.txt.gz 12.2 Mb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap