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Sample GSM1068572 Query DataSets for GSM1068572
Status Public on Jan 20, 2015
Title 02B-1353
Sample type RNA
Source name Pat1
Organism Homo sapiens
Characteristics tissue: skeletal muscle
gender: male
age: 1 year
sample type: Congenital Muscular Dystrophy
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from skeletal muscle biopsies with Qiagen (Qiagen, Hilden, Germany) RNeasy Fibrous Tissue mini kit following the manufacturer’s instructions
Label cy3
Label protocol RNA (500 ng) was labeled using Agilent's Low Input RNA Labeling Kit, which involves reverse transcribing the mRNA in the presence of T7-oligo-dT primer to produce cDNA and then in vitro transcribing with T7 RNA polymerase in the presence of Cy3-CTP
Hybridization protocol Labelled cRNAs were hybridised to the Agilent Human SurePrintGE, 8x60K according to manufacturer´s instructions
Scan protocol The arrays were washed, dried by centrifugation and scanned on an Agilent G2565BA microarray scanner at 100% PMT and 5 μm resolution
Description patient reference in corresponding publication: CMD2
Data processing Bioconductor limma package, using background correction (normexp) and quantiles normalization between arrays for final values
Submission date Jan 23, 2013
Last update date Jan 20, 2015
Contact name Susana Kalko
Organization name IDIBAPS
Department Bioinformatics Platform
Street address c/Rosello 153
City Barcelona
State/province BARCELONA
ZIP/Postal code 08036
Country Spain
Platform ID GPL13607
Series (1)
GSE43698 Muscle from patients with mitochondrial myopathies and congenital muscular dystrophies versus normal human muscle

Data table header descriptions
VALUE quantile normalized

Data table
4 7.953921641
5 6.721406976
6 6.356920971
7 10.81018877
8 8.562511873
9 6.061983486
10 6.350565475
11 6.063182588
12 9.886965077
13 10.17072868
14 7.481052147
15 6.723733282
16 6.041627484
17 6.71131566
18 6.084510609
19 6.205063411
20 9.814840079
21 8.821043262
22 6.128163608
23 6.098826798

Total number of rows: 62973

Table truncated, full table size 1089 Kbytes.

Supplementary file Size Download File type/resource
GSM1068572_06_MA_2300252800414336_Cy3_02B-1353_2_3.txt.gz 12.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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