|
| Status |
Public on Dec 01, 2013 |
| Title |
CebpaKO H3K27Me3 ChIP-seq 1 |
| Sample type |
SRA |
| |
|
| Source name |
Hematopoietic Stem Cells
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Hematopoietic Stem Cells
compartment: LSK (Lin- Sca-1+ Kit+)
antibody: H3K27Me3,Cell Signaling, C36B11
strain: C57BL/6 cebpa flox/flox;Mx1Cre
genotype/variation: knock out
|
| Growth protocol |
In vivo
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
FACS sorting on hematopoietic stem cell markers (Lin- Sca-1+ Kit+)
Kit: NEBNEXT ChIP-seq prep set1 (E6200S)
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Alignment to Mus genome Using bowtie v. 0.12.8 with standard parameters
Processing for coverage calculation: Bedtools version 2.16.2
Genome_build: mm9
Supplementary_files_format_and_content: Wiggle files containing read coverage
|
| |
|
| Submission date |
Dec 18, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Bo Torben Porse |
| E-mail(s) |
bo.porse@finselab.dk
|
| Phone |
+45 3545 6023
|
| Organization name |
University of Copenhagen/Rigshospitalet
|
| Department |
BRIC/Finsen Laboratory
|
| Lab |
Porse group
|
| Street address |
Ole Maaloes vej 5
|
| City |
Copenhagen |
| ZIP/Postal code |
2200 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL13112 |
| Series (1) |
| GSE43007 |
C/EBPa is required for self-renewal, lineage priming and maintenance of epigenetic configurations in hematopoietic stem cells |
|
| Relations |
| SRA |
SRX211466 |
| BioSample |
SAMN01831739 |
