|
| Status |
Public on Dec 31, 2013 |
| Title |
AZ-521_doxycycline_24h_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
AZ521 dnSox2 cells, induced, 24h
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: AZ521
genotype/variation: inducible dnSox2
cell type: gastric cancer cell line
treatment: doxycycline induced
time point: 24h
|
| Treatment protocol |
1 µg/ml doxycycline was added to induce expression of dnSox2.
|
| Growth protocol |
Minimum Essential Medium containing glutamine (MEM-GlutaMAXTM-I) (Gibco, Darmstadt, Germany) supplemented with 10% tetracycline-negative fetal bovine serum (PAA, Pasching, Austria) and 1% penicillin/streptomycin (Invitrogen, Darmstadt, Germany). All cells were maintained at 37 °C and 5% CO2 in a 95% air-humidified incubator.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
|
| Label |
biotin
|
| Label protocol |
Affymetrix WT cDNA Synthesis and Amplification and Terminal labeling kits.
|
| |
|
| Hybridization protocol |
Fluidics Station 450.
|
| Scan protocol |
Affymetrix GeneChip Scanner 3000.
|
| Description |
dox_24h_3
|
| Data processing |
RMA normalization by apt-probeset-summarize.exe from Affymetrix.
|
| |
|
| Submission date |
Dec 14, 2012 |
| Last update date |
Dec 31, 2013 |
| Contact name |
Stefan Krebs |
| E-mail(s) |
krebs-st@lmb.uni-muenchen.de
|
| Phone |
0049-89-2180 76715
|
| Organization name |
Ludwig-Maximilian University
|
| Department |
Gene Center
|
| Lab |
Lafuga Genomics
|
| Street address |
Feodor-Lynen-Str. 25
|
| City |
Munich |
| ZIP/Postal code |
81377 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE42937 |
Stem cell factor Sox2 regulates the tumorigenic potential in human gastric cancer cells |
|
