gender: male organism: Gorilla gorilla gorilla tissue: blood
Extracted molecule
genomic DNA
Extraction protocol
unknown
Label
Cy3, Cy5
Label protocol
Standard Illumina labelling protocol
Hybridization protocol
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
Scan protocol
Arrays were imaged using standard recommended Illumina scanner protocol
Description
A931
Data processing
The methylation value was set to null for probes with a detection p-value < 0.01. A strict filtering step was performed to remove divergent probes based on the number and location of mismatches with their target site in each species genome assembly tested. We only retained those probes that had either a perfect match, or had 1 or 2 mismatches in the first 45bp but no mismatches in the 3' 5bp closest to the CpG site being assayed. Beta values from the autosomal probes and chromosome X probes were quantile normalized separately; additionally, chromosome X probes for male and female were quantile normalized separately.
