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Series GSE41782 Query DataSets for GSE41782
Status Public on Jun 01, 2013
Title A genome-wide comparative study of DNA methylation in great apes
Platform organism Homo sapiens
Sample organisms Gorilla gorilla gorilla; Pan paniscus; Pongo pygmaeus; Pongo abelii; Homo sapiens; Pan troglodytes schweinfurthii; Pan troglodytes troglodytes; Pan troglodytes verus; Gorilla beringei graueri
Experiment type Methylation profiling by array
Summary DNA methylation is an epigenetic modification involved in regulatory processes such as cell differentiation during development, X-chromosome inactivation, genomic imprinting and susceptibility to complex diseases. These changes can be inherited through generations and likely have played an important role during human evolution. We performed a comparative analysis of CpG methylation patterns between humans and all great apes (chimpanzee, bonobo, gorilla and orangutan) on a total of 32 individuals.Our analysis identified ~1,000 genes with significantly altered methylation patterns among the great apes, including ~200 with a methylation pattern unique to humans. Analysis of genes with human-specific epigenetic patterns identified enrichments for several functional categories that are key for human-specific traits, such as advanced facial expressions or equilibrium related to bipedalism. We also found a highly significant positive correlation between the genomic distance genome-wide and the methylation levels. In a pairwise comparison, we observed that ~9% (~30,000) of CpGs assayed show significant methylation differences between human and chimpanzee, including promoter regions of 879 genes (11.9% of those tested), suggesting that epigenetic changes have occurred at high frequency during recent primate evolution and represent an important substrate for adaptive modification of genome function. Epigenetic changes among primates are highly enriched for sites showing intermediate DNA methylation levels might be related to regulatory elements, and we observed a significant relationship between changes in DNA methylation and gene expression across multiple different tissues. We also identified a significant positive relationship between the rate of coding variation within genes and alterations of promoter methylation, suggesting concerted evolution between protein sequence and gene regulation. Contrastingly, our analysis also identified scores of genes that are perfectly conserved at the amino acid level between human and chimp, yet which show significant epigenetic difference between these two species. We conclude that epigenetic alterations represent an important force during primate evolution and has been systematically under explored in evolutionary comparative genomics.
Overall design 32 samples analyzed in total: 9 humans, 5 chimpanzees, 6 bonobos, 6 gorillas and 6 orangutans. Blood-derived DNA methylation levels were assessed using Illumina Infinium HumanMethylation450 BeadChip. We performed a strict filtering step to remove divergent probes based on the number and location of mismatches with their target site in each species genome assembly tested, this resulted in the retention of 133,908 shared across all the species.
Contributor(s) Hernando-Herraez I, Marques-Bonet T, Sharp AJ, Garg P, Prado-Martinez J, Fernandez-Callejo M, Heyn H, Hvilsom C, Esteller M
Citation(s) 24039605
Submission date Oct 23, 2012
Last update date Mar 22, 2019
Contact name Irene Hernando
Organization name UPF/CSIC
Street address Dr Aiguader 88
City Barcelona
ZIP/Postal code 08003
Country Spain
Platforms (1)
GPL13534 Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482)
Samples (32)
GSM1024239 Pan troglodytes schweinfurthii A910
GSM1024240 Pan paniscus A914
GSM1024241 Pan paniscus A915
BioProject PRJNA178170

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE41782_RAW.tar 183.1 Mb (http)(custom) TAR
GSE41782_raw_and_processed.txt.gz 335.4 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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