|
| Status |
Public on Sep 28, 2012 |
| Title |
HudsonAlpha_ChipSeq_GM12878_RUNX3_(SC-101553)_v042211.1 |
| Sample type |
SRA |
| |
|
| Source name |
GM12878
|
| Organism |
Homo sapiens |
| Characteristics |
datatype: ChipSeq
datatype description: Chromatin IP Sequencing
antibody antibodydescription: Mouse monoclonal IgG, raised against amino acids 191-300 of recombinant RUNX3 of human origin. Antibody Target: RUNX3
antibody targetdescription: This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Multiple transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq)
antibody vendorname: Santa Cruz Biotechnology
antibody vendorid: sc-101553
controlid: SL6022
labexpid: SL8128,SL7898
softwareversion: MACS
cell sex: F
antibody: RUNX3_(SC-101553)
antibody antibodydescription: Mouse monoclonal IgG, raised against amino acids 191-300 of recombinant RUNX3 of human origin. Antibody Target: RUNX3
antibody targetdescription: This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Multiple transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq)
antibody vendorname: Santa Cruz Biotechnology
antibody vendorid: sc-101553
treatment: None
treatment description: No special treatment or protocol applies
protocol: v042211.1
protocol description: Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)
controlid: SL6022
labexpid: SL8128,SL7898
replicate: 1,2
softwareversion: MACS
|
| Biomaterial provider |
Coriell; http://ccr.coriell.org/Sections/Search/Search.aspx?PgId=165&q=GM12878
|
| Treatment protocol |
None
|
| Growth protocol |
GM12878_protocol.pdf
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer |
| |
|
| Data processing |
http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs
|
| |
|
| Submission date |
Sep 28, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
ENCODE DCC |
| E-mail(s) |
encode-help@lists.stanford.edu
|
| Organization name |
ENCODE DCC
|
| Street address |
300 Pasteur Dr
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305-5120 |
| Country |
USA |
| |
|
| Platform ID |
GPL9052 |
| Series (2) |
| GSE32465 |
Transcription Factor Binding Sites by ChIP-seq from ENCODE/HAIB |
| GSE51334 |
DNA replication-timing boundaries separate stable chromosome domains with cell-type-specific functions |
|
| Relations |
| SRA |
SRX190349 |
| BioSample |
SAMN01731778 |
| Named Annotation |
GSM1010893_hg19_wgEncodeHaibTfbsGm12878Runx3sc101553V0422111RawRep1.bigWig |
| Named Annotation |
GSM1010893_hg19_wgEncodeHaibTfbsGm12878Runx3sc101553V0422111RawRep2.bigWig |
