|
Status |
Public on Sep 28, 2012 |
Title |
HudsonAlpha_ChipSeq_A549_SP1_EtOH_0.02pct_v042211.1 |
Sample type |
SRA |
|
|
Source name |
A549
|
Organism |
Homo sapiens |
Characteristics |
datatype: ChipSeq datatype description: Chromatin IP Sequencing antibody antibodydescription: Sp1(E-3) is a mouse monoclonal antibody raised against amino acids 121-345 mapping near the N-terminus of Sp1 ofhuman origin. Recommended for detection of Sp1 of m, r and h origin by WB, IP, IF and ELISA. Antibody Target: SP1 antibody targetdescription: SP1 is a transcription factor that binds to GC box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites. It can interact with G/C-rich motifs from the serotonin receptor promoter. antibody vendorname: Santa Cruz Biotechnology antibody vendorid: sc-17824 controlid: SL6021 labexpid: SL5360,SL5701 softwareversion: MACS cell sex: M antibody: SP1 antibody antibodydescription: Sp1(E-3) is a mouse monoclonal antibody raised against amino acids 121-345 mapping near the N-terminus of Sp1 ofhuman origin. Recommended for detection of Sp1 of m, r and h origin by WB, IP, IF and ELISA. Antibody Target: SP1 antibody targetdescription: SP1 is a transcription factor that binds to GC box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites. It can interact with G/C-rich motifs from the serotonin receptor promoter. antibody vendorname: Santa Cruz Biotechnology antibody vendorid: sc-17824 treatment: EtOH_0.02pct treatment description: 1 h with 0.02% Ethanol (Myers) protocol: v042211.1 protocol description: Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers) controlid: SL6021 labexpid: SL5360,SL5701 replicate: 1,2 softwareversion: MACS
|
Biomaterial provider |
ATCC
|
Treatment protocol |
EtOH_0.02pct
|
Growth protocol |
A549_protocol.pdf
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs
|
|
|
Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
ChIP |
Instrument model |
Illumina Genome Analyzer |
|
|
Data processing |
http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeHaibTfbs
|
|
|
Submission date |
Sep 28, 2012 |
Last update date |
May 15, 2019 |
Contact name |
ENCODE DCC |
E-mail(s) |
encode-help@lists.stanford.edu
|
Organization name |
ENCODE DCC
|
Street address |
300 Pasteur Dr
|
City |
Stanford |
State/province |
CA |
ZIP/Postal code |
94305-5120 |
Country |
USA |
|
|
Platform ID |
GPL9052 |
Series (1) |
GSE32465 |
Transcription Factor Binding Sites by ChIP-seq from ENCODE/HAIB |
|
Relations |
SRA |
SRX190269 |
BioSample |
SAMN01731698 |
Named Annotation |
GSM1010813_hg19_wgEncodeHaibTfbsA549Sp1V0422111Etoh02RawRep1.bigWig |
Named Annotation |
GSM1010813_hg19_wgEncodeHaibTfbsA549Sp1V0422111Etoh02RawRep2.bigWig |