|
| Status |
Public on Sep 02, 2014 |
| Title |
Bos taurus_thymus_C17 |
| Sample type |
RNA |
| |
|
| Source name |
Bovine thymus samples collected at the slaughterhouse
|
| Organism |
Bos taurus |
| Characteristics |
tissue: thymus
breed: holstein
treatment: none
|
| Treatment protocol |
prednisolone 30 mg/head daily per os for 35 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extracted using the Invitrogen TRIzol reagent and purified according to the QiAgen RNeasy MinElute Cleanup Kit protocol for tissue samples
|
| Label |
Cyanine 3
|
| Label protocol |
Amplified and labeled according to Agilent One-Color Microarray-Based Gene Expression Analysis protocol
|
| |
|
| Hybridization protocol |
Hybridized according to Agilent One-Color Microarray-Based Gene Expression Analysis protocol
|
| Scan protocol |
Array was scanned according to standard Agilent protocol at a resolution of 5 microns modifying default setting to scan the slide twice at two different sensitivity settings (XDRHi 100% and XDRLo 10%)
|
| Description |
Sample of bovine thymus
|
| Data processing |
Data processed with FeatureExtraction v9.5 and normalized using cyclic lowess by R 2.11.1 statistical software
|
| |
|
| Submission date |
Sep 14, 2012 |
| Last update date |
Sep 02, 2014 |
| Contact name |
Serena Ferraresso |
| E-mail(s) |
serena.ferraresso@unipd.it
|
| Phone |
+39 049 8272506
|
| Organization name |
University of Padova
|
| Department |
Dept of Comparative Biomedicine and Food Science
|
| Street address |
Viale dell'Università
|
| City |
Legnaro (PD) |
| State/province |
Padova |
| ZIP/Postal code |
35020 |
| Country |
Italy |
| |
|
| Platform ID |
GPL7053 |
| Series (1) |
| GSE40884 |
Comparison of gene expression profile of thymus samples from prednisolone-treated and control cows. |
|
