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Series GSE97368 Query DataSets for GSE97368
Status Public on Sep 01, 2017
Title Gene expression profiling of low-dose irradiated human BM-MSCs
Organism Homo sapiens
Experiment type Expression profiling by array
Summary In bone marrow (BM), there are two different types of stem/progenitor cells. With respect to hematopoiesis, hematopoietic stem/progenitor cells (HPCs) produce mature blood cells and mesenchymal stromal/stem cells (MSCs) support this. The influence of exposure to low-dose radiation on human HPCs has been investigated, and generation of both immature and mature hematopoietic cells from human HPCs is compromised. On the other hand, the influence of exposure to low-dose radiation on MSCs is not known. This gene expression profiling was created for investigation how low-dose irradiation affects BM-MSCs genomically.
Overall design BM samples from healthy adult volunteers were purchased from AllCells (Emeryville, CA). Human BM-MSCs were isolated according to our previously published methods (Yao H, Stem Cells 2014;32:2245). BM-MSCs were exposed to 0.1 Gy of γ-radiation (Cesium137) at 0.8 Gy/min by a Gammacell Irradiator (Best Theratronics, Ontario, Canada). 24 hours after irradiation, total RNA were collected from the irradiated BM-MSCs.
Contributor(s) Fujishiro A, Miura Y, Ichinohe T
Citation Title: Effects of acute exposure to low-dose radiation on the characteristics of human bone marrow mesenchymal stromal/stem cells

Authors: Aya Fujishiro, Yasuo Miura, Masaki Iwasa, Sumie Fujii, Noriko Sugino,
Akira Andoh, Hideyo Hirai, Taira Maekawa and Tatsuo Ichinohe

Journal: Inflammation and Regeneration
Issue: 37:19
DOI: 10.1186/s41232-017-0049-2

Submission date Apr 04, 2017
Last update date Jan 09, 2018
Contact name Aya Fujishiro
Organization name Kyoto University Hospital
Department Transfusion Medicine and Cell Therapy
Street address Sakyo-ku
City Kyoto
ZIP/Postal code 606-8507
Country Japan
Platforms (1)
GPL17077 Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version)
Samples (8)
GSM2563142 Lot_A_0_Gy
GSM2563143 Lot_A_0.1_Gy
GSM2563144 Lot_B_0_Gy
BioProject PRJNA381533

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE97368_RAW.tar 24.4 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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