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| Status |
Public on Mar 01, 2019 |
| Title |
H3K27me3 enrichment in the supraorbital cranial mesenchyme with ectoderm upon the deletion of β-catenin |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
H3K27me3 enrichment in the supraorbital cranial mesenchyme with ectoderm upon the deletion of β-catenin
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| |
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| Overall design |
e13.5 supraorbital cranial mesenchyme was manually dissected. The sample collected consisted of all the tissue above the brain which included the cranial mesenchyme plus the ectoderm. Chromatin immunoprecipitation and sequencing (ChIP-seq) was then performed by Active Motif. 14 µg chromatin was immunoprecipitated with 4 µg rabbit anti-H3K27me3 (Millipore #07-449). Sequencing was performed on an Illumina NextSeq 500 producing 75-nucleotide, single end reads. Drosophila DNA was “spiked in” and used to normalize the number of reads in the samples. Reads were aligned using Bowtie2 to the mouse mm9 genome and peaks were called using Macs 1.4 at default settings.
contributor: Case Western Reserve University
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| |
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| Contributor(s) |
Atit R, Ferguson J |
| Citation(s) |
29223978 |
| |
| Submission date |
Mar 14, 2017 |
| Last update date |
Jul 29, 2019 |
| Contact name |
Radhika Paresh Atit |
| E-mail(s) |
rpa5@case.edu
|
| Phone |
2163688819
|
| Organization name |
Case Western Reserve University
|
| Department |
Biology
|
| Street address |
2074 Adelbert Road, Millis 316
|
| City |
Cleveland |
| State/province |
Ohio |
| ZIP/Postal code |
44106 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE96872 |
Supraorbital cranial mesenchyme with ectoderm upon the deletion of β-catenin |
|
| Relations |
| BioProject |
PRJNA379968 |
| SRA |
SRP102247 |
