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| Status |
Public on Feb 09, 2017 |
| Title |
Expression data from mir-35-41(nDf50) mutant embryos grown at 25 degrees, compared to wild type |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by array
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| Summary |
Gene expression in early animal embryogenesis is in large part controlled post-transcriptionally. Maternally-contributed microRNAs may therefore play important roles in early development. We have elucidated a major biological role of the nematode mir-35 family of maternally-contributed, essential microRNAs. We show that this microRNA family regulates the sex determination pathway at multiple levels, acting both upstream and downstream of her-1 to prevent aberrantly activated male developmental programs in hermaphrodite embryos. The predicted target genes that act downstream of the mir-35 family in this process, sup-26 and nhl-2, both encode RNA binding proteins, thus delineating a previously unknown post-transcriptional regulatory subnetwork within the well-studied sex determination pathway of C. elegans. Repression of nhl-2 by the mir-35 family is not only required for proper sex determination but also for viability, showing that a single microRNA target site can be essential. Since sex determination in C. elegans requires zygotic gene expression to read the sex chromosome karyotype, early embryos must remain gender-naïve; our findings show that the mir-35 family microRNAs act in the early embryo to function as a developmental timer that preserves naïveté and prevents premature deleterious developmental decisions.
The mir-35 family of microRNAs is essential for development. The mir-35-41(nDf50) allele deleted 7 of 8 mir-35 family members, and presents a hypomorphic phenotype in which embryonic lethality is temperature sensitive. To characterize the molecular phenotype associated with loss of mir-35 family function, we profiled gene expression in mir-35-41(nDf50) mutant embryos at both permissive (20) and restrictive (25) temperatures. (Refer to A microRNA family exerts maternal control on sex determination in C. elegans)
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| Overall design |
Mixed-stage embryos samples were obtained by hypochlorite treatment of mixed-stage C. elegans cultures. Cultures were grown on NGM supplemented with chicken egg yolk, and upshifted from 20 to 25 degrees 24h before harvest.
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| Contributor(s) |
McJunkin K, Ambros V |
| Citation(s) |
28279983 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 GM034028 |
Genetic control of animal development |
UNIV OF MASSACHUSETTS MED SCHOOL |
Victor Ambros |
| F32 GM097895 |
Genetic analysis of post-transcriptional modulators of microRNAs in C. elegans |
UNIV OF MASSACHUSETTS MED SCHOOL |
Katherine McJunkin |
| K99 GM113063 |
Molecular Mechanisms of microRNA and miRISC turnover |
UNIV OF MASSACHUSETTS MED SCHOOL |
Katherine McJunkin |
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| Submission date |
Feb 08, 2017 |
| Last update date |
May 19, 2017 |
| Contact name |
Katherine McJunkin |
| E-mail(s) |
mcjunkin@nih.gov
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| Phone |
3048811944
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| Organization name |
National Institutes of Health
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| Street address |
50 South Drive, Building 50 Room 3148
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| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL200 |
[Celegans] Affymetrix C. elegans Genome Array |
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| Samples (6)
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| GSM2481234 |
mir-35-41(nDf50) embryo, 25 degrees, rep 1 |
| GSM2481235 |
mir-35-41(nDf50) embryo, 25 degrees, rep 2 |
| GSM2481236 |
mir-35-41(nDf50) embryo, 25 degrees, rep 3 |
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| This SubSeries is part of SuperSeries: |
| GSE94704 |
A microRNA family exerts maternal control on sex determination in C. elegans |
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| Relations |
| BioProject |
PRJNA373803 |
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