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Series GSE94128 Query DataSets for GSE94128
Status Public on Feb 21, 2018
Title Transcriptome profile in INT cells modulated by Cryptosporidium parvum RNA transcripts
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Cryptosporidium parvum is an important opportunistic parasite pathogen for immunocompromised individuals and a common cause of diarrhea in young children in developing countries. Certain parasite molecules can be delivered into host epithelial cells and may act as effector molecules for parasite intracellular development. This study aims to measure the impact of transfection of two parasite low-protein coding potential RNA transcripts, cdg2_FLc_0220 and cdg7_FLc_1000, on the transcriptome profile in intestinal epithelial cells. Human intestinal epithelial INT (FHs 74 Int) cells were grown to 80% confluence and transfected with the cdg2_FLc_0220 or cdg7_FLc_1000full-length or the empty vector for 48h. Total RNA was collected for the genome-wide analysis. The Agilent SurePrint G3 Human Gene Expression Microarray (G4851B) was used for the genome-wide analysis, which provides full coverage of genes and transcripts with the most up-to-date content, including mRNAs and lincRNAs (
Overall design The FHs 74 Int were grown to 80% confluence for three groups: pcDNA3.1(+) empty vector (Group CP-A, cells transfected with pcDNA3.1(+) empty vector), pcDNA3.1(+)_cdg2_FLc_0220 full-length (Group CP-B, cells transfected with pcDNA3.1(+)_cdg2_FLc_0220 full-length ) and pcDNA3.1(+)_cdg7_FLc_1000 full-length (Group CP-C, cells transfected with pcDNA3.1(+)_cdg7_FLc_1000 full-length ). 48h later, total RNAs were prepared with the RNeasy Mini kit (Qiagen) according to the manufacturer’s instruction.
Contributor(s) Chen X
Citation(s) 29426472, 28961856
Submission date Jan 27, 2017
Last update date Jul 08, 2019
Contact name Xianming Chen
Organization name creighton university
Street address 2500 california plaza
City Omaha
State/province NE
ZIP/Postal code 68178
Country USA
Platforms (1)
GPL17077 Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version)
Samples (9)
GSM2470547 pcDNA3.1(+) empty vector (CP-A1)
GSM2470548 pcDNA3.1(+) empty vector (CP-A2)
GSM2470549 pcDNA3.1(+) empty vector (CP-A3)
BioProject PRJNA369033

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE94128_RAW.tar 110.9 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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