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| Status |
Public on Jun 09, 2017 |
| Title |
Transcriptome analysis of dominant-negative Brd4 mutants identifies Brd4-specific target genes of BET inhibitor JQ1 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We analyzed anti-proliferative dominant-negative Brd4 mutants that compete with the function of distinct Brd4 domains. We used these Brd4 mutants to compare the Brd4-specific transcriptome with the transcriptome of JQ1 treated cells.
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| Overall design |
We performed polyA RNA-seq of Raji cells expressing Brd4 constructs f3 and f9 (dnBrd4_f3/f9) and Raji cell expressing the luciferase control vector treated with JQ1 (luc_JQ1) or DMSO as control (luc_DMSO).
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| Contributor(s) |
Decker T, Kluge M, Krebs S, Shah N, Blum H, Friedel CC, Eick D |
| Citation(s) |
28490802 |
| |
| Submission date |
Dec 15, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Caroline Friedel |
| Organization name |
Ludwig-Maximilians-Universität München
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| Department |
Institut für Informatik
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| Street address |
Amalienstr. 17
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| City |
München |
| ZIP/Postal code |
80333 |
| Country |
Germany |
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| Platforms (1) |
| GPL18460 |
Illumina HiSeq 1500 (Homo sapiens) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA357609 |
| SRA |
SRP095194 |
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