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Series GSE9077 Query DataSets for GSE9077
Status Public on Apr 01, 2008
Title Expression profiles of immortal lung fibroblasts
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Activation of telomerase often endows cancer cells, but rarely normal somatic cells, with immortality. Especially, fetal lung fibroblasts are known to be hardly immortalized by TERT overexpression. We here established an immortal non-transformed lung fibroblast cell line only by TERT transfection, as well as an immortal transformed cell line by transfection of TERT and SV40 early antigens. Comparing the expression profiles of these cell lines with those of mortal cell strains with elongated lifespan after TERT transfection, 51 genes, including 19 upregulated and 32 downregulated, were explored to be the candidates responsible for regulation of cellular proliferation of lung fibroblasts. These included the genes previously reported to be involved in cellular proliferation, transformation, or self-renewal capacity, and those highly expressed in lung tissues obtained from patients with idiopathic pulmonary fibrosis or hypersensitivity pneumonitis. This set of lung fibrobrast cell lines/strains of identical genetic background with different proliferative capacity, mortal and immortal non-transformed fibroblasts may become useful model cells for research on lung fibroblast growth regulation and the candidate genes explored in this study may provide promising biomarkers or molecular targets of pulmonary fibrosis.
Keywords: Cell type comparison
Overall design We analyzed 6 arrays with Affymetrix, 4 arrays with Agilent, and 7 arrays with CodeLink oligonucleotide microarrays for lung fibroblasts with various lifespan with or without transfection of TERT and/or SV40 early antigens.
Contributor(s) Hiyama K, Hiyama E, Tanimoto K, Nishiyama M
Citation(s) 18716925
Submission date Sep 18, 2007
Last update date Mar 25, 2019
Contact name Keiko Hiyama
Phone 81-82-257-5841
Fax 81-82-256-7105
Organization name Hiroshima University
Department Research Institute for Radiation Biology and Medicine
Lab Dept. Translational Cancer Research
Street address 1-2-3 Kasumi, Minami-ku
City Hiroshima
State/province Hiroshima
ZIP/Postal code 734-8553
Country Japan
Platforms (3)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
GPL1928 CodeLink Human 20K ver4.1
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (17)
GSM230006 TIG-1-45, parent
GSM230007 TERT6-65, TERT transfected
GSM230008 TERT6-85, TERT transfected
BioProject PRJNA102595

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE9077_RAW.tar 86.2 Mb (http)(custom) TAR (of CEL, TXT)
Processed data included within Sample table

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