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Sample GSM230078 Query DataSets for GSM230078
Status Public on Apr 01, 2008
Title TERT6-217, TERT transfected, Agilent
Sample type RNA
Source name human fetal lung fibroblast TIG-1 clone TERT6 transfected with TERT
Organism Homo sapiens
Characteristics human fetal lung fibroblast TIG-1 clone TERT6 at 217 PDL transfected with TERT
Biomaterial provider Health Science Research Resources Bank, Tokyo, Japan
Treatment protocol TERT expression vector was transfected to TIG-1 and isolated as a G418 resistant clone. Exponentially growing cultured cells were collected using trypsin followed by rinse with PBS, and freezed until use.
Growth protocol Cells were cultured in Dulbecco's modified Eagle's minimal essential medium (DMEM; Sigma-Aldrich Japan, Tokyo, Japan) containing 10% heat-inactivated fetal bovine serum (FBS; BioWhittaker, Verviers, Belgium) in a humidified atmosphere of 5% CO2.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from the frozen cultured cell pellets using QIAGEN RNeasy mini kit (QIAGEN, Inc., Valencia, CA), respectively, according to the manufacturer's protocols. The quality of the RNA was checked using Agilent Technologies 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).
Label Cy3
Label protocol The cDNA was generated from 0.6 microgram of total RNA using reverse transcriptase and a T7 primer using Agilent Low RNA Input Linear Amplification Kit PLUS, One-color (Agilent). cRNA (complementary RNA) was generated via an in vitro transcription reaction using T7 RNA polymerase and cyanine 3-labeled CTP
Hybridization protocol The synthesized 1.5 microgram of cRNA, quantified by spectrometry and qualified using an Agilent 2100 Bioanalyzer, was then fragmented and hybridized to each array. After 17hr hybridization, arrays were washed according to the manufacturer's recommended protocol.
Scan protocol Each microarray was scanned using an Agilent DNA Microarray Scanner.
Description immortal lifespan
Data processing Expression levels were quantified by Agilent Feature Extraction software ver. 9.1. and normalized to the median expression value of the whole array spots using GeneSpringTM GX (Agilent Technologies).
Submission date Sep 18, 2007
Last update date Aug 14, 2011
Contact name Keiko Hiyama
Phone 81-82-257-5841
Fax 81-82-256-7105
Organization name Hiroshima University
Department Research Institute for Radiation Biology and Medicine
Lab Dept. Translational Cancer Research
Street address 1-2-3 Kasumi, Minami-ku
City Hiroshima
State/province Hiroshima
ZIP/Postal code 734-8553
Country Japan
Platform ID GPL4133
Series (1)
GSE9077 Expression profiles of immortal lung fibroblasts

Data table header descriptions
VALUE normalized signal intensity

Data table
1 3.06E+05
2 1.37E+01
3 1.09E+02
4 1.04E+02
5 1.10E+02
6 1.04E+02
7 1.11E+02
8 1.05E+02
9 1.01E+02
10 1.06E+02
11 1.03E+02
12 9.93E+02
13 1.89E+02
14 1.51E+03
15 1.07E+02
16 1.51E+04
17 1.08E+02
18 3.11E+02
19 5.82E+04
20 8.84E+01

Total number of rows: 45015

Table truncated, full table size 648 Kbytes.

Supplementary file Size Download File type/resource
GSM230078.txt.gz 6.6 Mb (ftp)(http) TXT
Processed data included within Sample table

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