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Series GSE89779 Query DataSets for GSE89779
Status Public on Nov 12, 2016
Title Development of diagnostic exosomal biomarkers for the detection of invasive non-functional pituitary adenomas (NFPAs)
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The incomplete surgical resection of invasive non-functional pituitary adenomas (iNFPAs) carries the increased risk of complications and requires adjuvant radiotherapy and medications. Thus, the molecular mechanisms and markers of invasiveness must be identified to guide the management of NFPA patients. This study explores the proteomic and transcriptomic variations of invasive and non-invasive NFPAs and other types of pituitary adenomas and evaluates the genetic markers in the exosome related to iNFPAs. The exosome from invasive and non-invasive NFPAs, prolactinomas (PRLs), growth hormone–secreting adenomas (GHs), adrenocorticotropic hormone-secreting adenomas (ACTHs), and normal pituitary tissue were analyzed. We confirmed that elevated matrix metalloproteinase-1 (MMP1) expression and its production in the exosome (exo-MMP1) are correlated with the invasive characteristics of NFPA. To investigate the molecular mechanism underlying the role of exo-MMP1 in invasiveness, we analyzed the effects of MMP1 on cell migration, cell growth and tumor angiogenesis. After transfection of MMP1 or a shRNA expression vector into NFPA cells, we obtained the associated exosome and observed that the altered expression and production of MMP1 in the exosome was significantly synchronized with the transduction of NFPA cells. In addition, the enrichment of MMP1 in the exosome promoted cell migration, cell growth and tumor angiogenesis via protease-activated receptor-1 (PAR1) signaling in recipient cells. Thus, these data demonstrate that MMP1 plays an important role in tumor invasion and angiogenesis and show that an exosome-mediated regulatory pathway for MMP1 may represent a target for therapeutic treatment.
Overall design This study explores the transcriptomic variations of invasive (n=1) and non-invasive NFPAs (n=1) in the exosome.
Contributor(s) Ren Y, Wang R
Citation(s) 35085587
Submission date Nov 11, 2016
Last update date Jan 28, 2022
Contact name Yuan Ren
Phone +8615810639625
Organization name Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College
Department Neurosurgery
Street address Dongcheng district, No.1, beishuaifuyuan
City Beijing
State/province Beijing
ZIP/Postal code 100050
Country China
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (2)
GSM2388547 Exosome of invasive NFPAs
GSM2388548 Exosome of non-invasive NFPAs
BioProject PRJNA353245
SRA SRP093258

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Supplementary file Size Download File type/resource
GSE89779_normalized_mRNA_exp.xlsx 2.5 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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