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| Status |
Public on Nov 12, 2016 |
| Title |
Exosome of non-invasive NFPAs |
| Sample type |
SRA |
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| Source name |
exosome
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| Organism |
Homo sapiens |
| Characteristics |
sample type: non-invasive non-functional pituitary adenomas
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| Extracted molecule |
total RNA |
| Extraction protocol |
Total exosomal RNA was extracted using the HiPure Serum/Plasma miRNA Kit (Magen R4317). RNA quality was assessed with the Qubit 3.0 (Thermo Scientific, Rockford, IL, USA) and the Agilent 2200 TapeStation. The NEBNext® Ultra™ RNA Library Prep Kit (Beverly, MA, USA) for Illumina was used to prepare an RNA-seq library. Finally, library sequencing was conducted on an Illumina HiSeq™ 3000 system.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 3000 |
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| Data processing |
Total exosomal RNA was extracted using the HiPure Serum/Plasma miRNA Kit (Magen R4317). RNA quality was assessed with the Qubit 3.0 (Thermo Scientific, Rockford, IL, USA) and the Agilent 2200 TapeStation. The NEBNext® Ultra™ RNA Library Prep Kit (Beverly, MA, USA) for Illumina was used to prepare an RNA-seq library. Finally, library sequencing was conducted on an Illumina HiSeq™ 3000 system.
Genome_build: Sequence data were mapped to a human reference genome hg19 with TopHat v2.0.13. gfold v1.1.2 was subsequently employed to count the number of reads mapped to each gene.
Supplementary_files_format_and_content: Differential expression was assessed via DEGseq using RPKM as the input. DEGs were chosen according to the criteria of a > 2-fold change and adjusted P-value < 0.05.
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| Submission date |
Nov 11, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Yuan Ren |
| E-mail(s) |
15810639625@163.com
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| Phone |
+8615810639625
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| Organization name |
Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College
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| Department |
Neurosurgery
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| Street address |
Dongcheng district, No.1, beishuaifuyuan
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| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100050 |
| Country |
China |
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| Platform ID |
GPL21290 |
| Series (1) |
| GSE89779 |
Development of diagnostic exosomal biomarkers for the detection of invasive non-functional pituitary adenomas (NFPAs) |
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| Relations |
| BioSample |
SAMN06013362 |
| SRA |
SRX2343057 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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