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Series GSE89610 Query DataSets for GSE89610
Status Public on Nov 09, 2016
Title Impact of IL-27 on hepatocyte antiviral gene expression and function
Organism Homo sapiens
Experiment type Expression profiling by array
Summary IL-27 has been shown to play a role in modulating functions of diverse cell types like Th1, Th2, NK and B cells demonstrating both pro and anti-inflammatory roles. It is also capable of inducing STAT1, STAT3 and ISG's. To investigate the role of IL-27 on hepatocytes, Huh7.5 cells were treated with Il-27 and analysed the differentially expressed genes by microarray analysis.
Microarray analysis showed that the upregulated genes belonged to antigen presentation and DNA replication pathways and involved strong upregulation of CXCL-10 a gene associated with liver inflammation.
 
Overall design 28 Huh7.5 Samples stimulated with IFNA, IL10, IL17, IL22, IL27 and IL28 for 24 and 72 hrs.
 
Contributor(s) Ramamurthy N, Boninsegna S, Adams R, Sahgal N, Lockstone H, Baban D, Marchi E, Klenerman P
Citation(s) 28058287, 29534310
Submission date Nov 07, 2016
Last update date Nov 14, 2018
Contact name Timothy Hinks
E-mail(s) timothy.hinks@ndm.ox.ac.uk
Organization name University of Oxford
Department NDM Experimental Medicine - New College
Street address John Radcliffe Hospital, Headley Way, Oxford
City Oxford
State/province United Kingdom
ZIP/Postal code OX3 9DU
Country United Kingdom
 
Platforms (1)
GPL6244 [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version]
Samples (28)
GSM2385322 Mock Stimulated Huh7.5 cells 1
GSM2385323 Huh7.5 cells stimulated with IL22 for 24 hrs 1
GSM2385324 Huh7.5 cells stimulated with IL10 for 72 hrs 1
Relations
BioProject PRJNA352859

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE89610_RAW.tar 111.3 Mb (http)(custom) TAR (of CEL)
GSE89610_non-normalized.txt.gz 4.9 Mb (ftp)(http) TXT
Processed data included within Sample table

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