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| Status |
Public on Nov 18, 2016 |
| Title |
Microarray Samples for shTP63 in HNSCC Cells to Investigate How ACTL6A and p63 Activate Hippo-YAP in SCC |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Loss-of-function mutations in SWI/SNF chromatin remodeling subunit genes are observed in many cancers, but an oncogenic role for SWI/SNF is not well established. Here we reveal that ACTL6A, encoding a SWI/SNF subunit linked to stem and progenitor cell function, is frequently co-amplified and highly expressed together with the p53 family member p63 in head and neck squamous cell carcinoma (HNSCC). ACTL6A and p63 physically interact and cooperatively control a transcriptional program that promotes proliferation and suppresses differentiation, in part through activation of the Hippo-YAP pathway via regulators including WWC1. Consequently, loss of ACTL6A or p63 in tumor cells induces YAP phosphorylation and inactivation, associated with growth arrest and terminal differentiation, all phenocopied by WWC1 overexpression. In vivo, ectopic ACTLC6A/p63 expression promotes tumorigenesis, while ACTL6A expression and YAP activation are highly correlated in primary HNSCC and predict poor patient survival. Thus, ACTL6A and p63 collaborate as oncogenic drivers in HNSCC.
Gene expression profiling of HNSCC cells with and without ablated endogenous p63 via lentiviral shRNA
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| Overall design |
Examination of mRNA levels in head and neck squamous cell carcinoma (HNSCC) cell lines JHU-029 and FaDU after treatment with shRNA for a vector control or TP63 with 3 replicates for each cell line and treatment
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| Contributor(s) |
Saladi SV, Ellisen LW, Ross KN |
| Citation(s) |
28041841 |
| |
| Submission date |
Oct 17, 2016 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Kenneth N Ross |
| E-mail(s) |
Kenneth.Ross@dfci.harvard.edu
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| Organization name |
Dana-Farber Cancer Institute
|
| Department |
Pediatric Oncology
|
| Street address |
450 Brookline Ave., Rm M640
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE88861 |
Cells to Investigate How ACTL6A and p63 Activate Hippo-YAP in SCC |
|
| Relations |
| BioProject |
PRJNA348822 |
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