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Series GSE87365 Query DataSets for GSE87365
Status Public on Sep 27, 2016
Title ERBB3 and NGFR mark distinct skeletal muscle progenitor cells in human development enabling enrichment and maturation of hPSC muscle
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Abstract: Human pluripotent stem cells (hPSCs) can be directed to differentiate into skeletal muscle progenitor cells (SMPCs). However, the myogenic potential of hPSC-SMPCs compared to human fetal or adult satellite cells (Scs) remains unclear. This study demonstrates hPSC-SMPCs derived by commonly used protocols are functionally less mature than freshly-isolated human fetal or adult Scs. We utilized RNA-SEQ of human fetal Scs to identify differentially expressed genes including NGFR, ERBB3, which enriched for MYOD+ or PAX7+ cells. Furthermore, inhibition of TGFβ (TGFβi) improved hPSC-muscle maturation in three independent lines as measured by TEM and expression of myosins. Engraftment of CRISPR/Cas9-corrected Duchenne Muscular Dystrophy (DMD) hiPSC-SMPC subpopulations treated with TGFβi in vivo, increased dystrophin-positive fibers to levels of engrafted cultured fetal Scs in mdx mouse models of DMD. This work provides the first characterization of the developmental status of hPSC-SMPCs and identifies candidates to enable robust myogenic activity in vitro and in vivo.
Overall design Profiled transcriptomes of uncultured fetal Scs, cultured fetal muscle cells, and differentiated fetal myotubes in comparison to day 50 NCAM+ hPSC-SMPCs and SMPC-derived myotubes by RNA-SEQ.
Contributor(s) Hicks M, Eskin A
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Submission date Sep 26, 2016
Last update date May 15, 2019
Contact name Ascia Eskin
Organization name UCLA
Department Human Genetics
Lab Stanley Nelson
Street address 695 Charles Young Drive South Gonda 5554
City Los Angeles
State/province California
ZIP/Postal code 90095-7088
Country USA
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (10)
GSM2328841 1_Fresh_W17_0_Imm_Sort_S6
GSM2328842 2_Fresh_W17_1_Imm_Sort_S5
GSM2328843 3_Fresh_W17_2_p0_S10
BioProject PRJNA344494
SRA SRP090475

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Supplementary file Size Download File type/resource
GSE87365_gene_exp_diff_fc_yes_reg_Fetal_myotubes_vs_HPSC_myotubes_and_FACS_sort.xls.gz 91.3 Kb (ftp)(http) XLS
GSE87365_gene_exp_diff_fc_yes_reg_Fetal_stem_cells_and_FACS_sort_vs_Fetal_stem_cells.xls.gz 445.7 Kb (ftp)(http) XLS
GSE87365_gene_exp_diff_fc_yes_reg_Fetal_stem_cells_and_FACS_sort_vs_HPSC_stem_cells_and_FACS_sort.xls.gz 78.6 Kb (ftp)(http) XLS
GSE87365_gene_exp_diff_fc_yes_reg_Fetal_stem_cells_vs_Fetal_myotubes.xls.gz 291.6 Kb (ftp)(http) XLS
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