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| Status |
Public on Sep 27, 2016 |
| Title |
ERBB3 and NGFR mark distinct skeletal muscle progenitor cells in human development enabling enrichment and maturation of hPSC muscle |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Abstract: Human pluripotent stem cells (hPSCs) can be directed to differentiate into skeletal muscle progenitor cells (SMPCs). However, the myogenic potential of hPSC-SMPCs compared to human fetal or adult satellite cells (Scs) remains unclear. This study demonstrates hPSC-SMPCs derived by commonly used protocols are functionally less mature than freshly-isolated human fetal or adult Scs. We utilized RNA-SEQ of human fetal Scs to identify differentially expressed genes including NGFR, ERBB3, which enriched for MYOD+ or PAX7+ cells. Furthermore, inhibition of TGFβ (TGFβi) improved hPSC-muscle maturation in three independent lines as measured by TEM and expression of myosins. Engraftment of CRISPR/Cas9-corrected Duchenne Muscular Dystrophy (DMD) hiPSC-SMPC subpopulations treated with TGFβi in vivo, increased dystrophin-positive fibers to levels of engrafted cultured fetal Scs in mdx mouse models of DMD. This work provides the first characterization of the developmental status of hPSC-SMPCs and identifies candidates to enable robust myogenic activity in vitro and in vivo.
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| Overall design |
Profiled transcriptomes of uncultured fetal Scs, cultured fetal muscle cells, and differentiated fetal myotubes in comparison to day 50 NCAM+ hPSC-SMPCs and SMPC-derived myotubes by RNA-SEQ.
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| Contributor(s) |
Hicks M, Eskin A |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Sep 26, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Ascia Eskin |
| Organization name |
UCLA
|
| Department |
Human Genetics
|
| Lab |
Stanley Nelson
|
| Street address |
695 Charles Young Drive South Gonda 5554
|
| City |
Los Angeles |
| State/province |
California |
| ZIP/Postal code |
90095-7088 |
| Country |
USA |
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| Platforms (1) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA344494 |
| SRA |
SRP090475 |
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