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Sample GSM2328845 Query DataSets for GSM2328845
Status Public on Sep 27, 2016
Title 5_FW17_0_N2_d10_S8
Sample type SRA
Source name Fetal limb muscle
Organism Homo sapiens
Characteristics tissue: Fetal limb muscle
cell status: Fetal Myotubes
age: human week 17
rna collection post facs: No FACS
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using Qiagen's Rneasy Micro Kit.
RNA-Seq libraries were constructed with Roche's KAPA Stranded RNA-Seq Library Preparation Kit, using standard protocols
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 3000
Description RNA from these samples were collected from primary human muscle tissue after being differentiated to form myotubes for 7 days in N2 media.
processed data file: gene_exp_diff_fc_yes_reg_Fetal_myotubes_vs_HPSC_myotubes_and_FACS_sort.xls, gene_exp_diff_fc_yes_reg_Fetal_stem_cells_vs_Fetal_myotubes_MH_02022016.xls
Data processing Illumina Casava software used for basecalling.
Reads were aligned to hg19 genome using TopHat v2.0.8 with parameters -G (using Ensembl GRCh37 GTF) and --no-novel-juncs.
Transcripts were assembled using Cufflinks v2..1. Cuffdiff was used to compare samples, using Ensembl GRCh37 GTF
Genome_build: hg19
Supplementary_files_format_and_content: Excel Cuffdif result files include FPKM values for each Sample, as well as q-value and fold change.
Submission date Sep 26, 2016
Last update date May 15, 2019
Contact name Ascia Eskin
Organization name UCLA
Department Human Genetics
Lab Stanley Nelson
Street address 695 Charles Young Drive South Gonda 5554
City Los Angeles
State/province California
ZIP/Postal code 90095-7088
Country USA
Platform ID GPL21290
Series (1)
GSE87365 ERBB3 and NGFR mark distinct skeletal muscle progenitor cells in human development enabling enrichment and maturation of hPSC muscle
BioSample SAMN05826477
SRA SRX2191448

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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