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| Status |
Public on Sep 18, 2016 |
| Title |
NIPI-3 regulates the expression of C. elegans immune genes |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by array
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| Summary |
Many pathogens secrete toxins that target key host processes resulting in the activation of immune pathways. The secreted Pseudomonas aeruginosa toxin Exotoxin A (ToxA) disrupts intestinal protein synthesis which triggers the induction of a subset of P. aeruginosa-response genes in the nematode Caenorhabditis elegans. We found that losing one ToxA-induced C. elegans gene, the Tribbles pseudokinase ortholog nipi-3, results in hypersusceptibility to both P. aeruginosa and ToxA. We determined that NIPI-3 mediates the post-developmental expression of intestinal immune genes and proteins and primarily functions in parallel to known immune pathways, including p38 PMK-1 MAPK signaling. Here we present the microarray data that was used to determine that (1) nipi-3 regulates immune gene expression and that (2) nipi-3 and pmk-1 regulate non-overlapping gene sets consistent with them functioning in parallel.
We used microarray analysis to identify the genes regulated by nipi-3 and pmk-1 at the L4 stage.
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| Overall design |
There are nine samples total that comprise three biological replicates of L4 C. elegans animals grown at 20C on OP50. The following genotypes were compared: wild type (N2), nipi-3(fr4), pmk-1(km25).
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| Contributor(s) |
McEwan DL, Ausubel FM |
| Citation(s) |
27927200 |
| |
| Submission date |
Sep 17, 2016 |
| Last update date |
Dec 19, 2016 |
| Contact name |
Deborah L McEwan |
| Organization name |
MGH
|
| Street address |
185 Cambridge St CPZN 7250
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL200 |
[Celegans] Affymetrix C. elegans Genome Array |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA343331 |
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