|
| Status |
Public on Mar 28, 2008 |
| Title |
Mouse embryonic stem cell line R1 differentiation study |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Cell line R1 (Mouse embryonic stem cell line) was cultured on 3 different conditions for differentiation study. The experiment ran for 7 days and samples were taken every 24hrs. Afterward, samples on days 0,3,5 and 7 were processed for microarray analysis.
The conditions were the differentiation of the stem cells for 7 days through a)formation of embryoid bodies, b)culture on a gelatin coated surface and c) culture on a matrigel coated surface.
Keywords: Time course
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| |
|
| Overall design |
10 samples, considering: days 3, 5 and 7 for each of the 3 differentiating conditions and one for day zero. Technical replicates were made every three sample points. 3 Complete biological replicates were performed.
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| |
|
| Contributor(s) |
Sepulveda DE, Papoutsakis ET, Asenjo JA |
| Citation(s) |
18433312 |
| |
| Submission date |
Jul 24, 2007 |
| Last update date |
Dec 06, 2012 |
| Contact name |
Dario Sepulveda |
| E-mail(s) |
dsepulve@ing.uchile.cl
|
| Organization name |
Universidad de Chile
|
| Department |
Chemical Engineering and Biotechnology
|
| Lab |
CIByB
|
| Street address |
Beacuhef 861
|
| City |
Santiago |
| State/province |
RM |
| ZIP/Postal code |
8370456 |
| Country |
Chile |
| |
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| Platforms (1) |
| GPL891 |
Agilent-011978 Mouse Microarray G4121A (Feature Number version) |
|
| Samples (36)
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|
| Relations |
| BioProject |
PRJNA101719 |
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