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| Status |
Public on May 24, 2017 |
| Title |
Grape seed extracts-mediated lipid mobility in C. elegans |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by array
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| Summary |
Oligomeric proanthocyanidins (OPCs) reduce triglycerides in the nematode C. elegans. Lipase was strongly inhibited in vitro accompanied by the reduction of total triglyceride storage capacity in vivo; Lipophilic staining was also attenuated in wild type worms and high-fat mutants exposed to OPCs. Apart from biochemical analyses, lipid metabolism was also genetically regulated, emphasizing the necessity to study underlying regulation mechanisms in intact animals. To gain a deeper insight into the potential gene targets of purified oligomeric proanthocyanidin trimer gallate (pOPC7), a binary microarray assay was carried out with wild type N2 populations continuously exposed to a bacterial diet with or without pOPC7.
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| Overall design |
The global transcriptome was compared in wild type N2 raised in the presence or absence of oligomeric proanthocyanidin trimer gallate (pOPC7) extracted from Vitis vinifera grape seeds at the concentration of 2 µg/mL for 48 hours (L1 to L4 stage).
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| Contributor(s) |
Nie Y, Schuster E, Arno M, Sturzenbaum SR |
| Citation(s) |
28760744 |
| |
| Submission date |
May 25, 2016 |
| Last update date |
Aug 23, 2017 |
| Contact name |
Stephen Sturzenbaum |
| E-mail(s) |
stephen.sturzenbaum@kcl.ac.uk
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| Organization name |
King's College London
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| Department |
Analytical, Environmental and Forensic Sciences
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| Street address |
150 Stamford Street
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| City |
London |
| ZIP/Postal code |
SE1 9NH |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL200 |
[Celegans] Affymetrix C. elegans Genome Array |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA322749 |
