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| Status |
Public on Apr 01, 2016 |
| Title |
Cumulus cell gene expression associated with pre-ovulatory acquisition of developmental competence in bovine oocytes |
| Organism |
Bos taurus |
| Experiment type |
Expression profiling by array
|
| Summary |
This study investigated the cumulus cell (CC) transcriptomic changes during the oocyte developmental competence acquisition period.
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| Overall design |
Six dairy cows were used for 24 oocyte collections and received FSH twice daily over 3 days, followed by FSH withdrawal for 20, 44, 68 and 92 h in four different oestrous cycles for each of the six cows. Half of the cumulus–oocyte complexes were subjected to in vitro maturation, fertilisation and culture to assess blastocyst rate. The other half of the CC underwent microarray analysis and qRT-PCR.
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| |
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| Contributor(s) |
Bunel A, Nivet A, Blondin P, Vigneault C, Richard FJ, Sirard M |
| Citation(s) |
23827322 |
| |
| Submission date |
Mar 31, 2016 |
| Last update date |
Jul 01, 2016 |
| Contact name |
Marc-André Sirard |
| E-mail(s) |
Marc-Andre.Sirard@fsaa.ulaval.ca
|
| Organization name |
Université Laval
|
| Department |
Sciences Animales
|
| Street address |
Offfice 2732, 2440 Hochelaga Blvd.
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| City |
Québec City |
| State/province |
Quebec |
| ZIP/Postal code |
G1V 0A6 |
| Country |
Canada |
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| Platforms (1) |
| GPL13226 |
Agilent-028298 Embryogene Bovine 45K microarray |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA316958 |
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