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| Status |
Public on Nov 25, 2015 |
| Title |
The tissue-engineered human cornea as a model to study expression of matrix metalloproteinases during corneal wound healing |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Corneal injuries remain a major cause of consultation in the ophthalmology clinics worldwide. Repair of corneal wounds is a complex mechanism that involves cell death, migration, proliferation, differentiation, and extracellular matrix (ECM) remodeling. In the present study, we used a tissue-engineered, two-layers (epithelium and stroma) human cornea as a biomaterial to study both the cellular and molecular mechanisms of wound healing. Gene profiling on microarrays revealed important alterations in the pattern of genes expressed by tissue-engineered corneas in response to wound healing. Expression of many MMPs-encoding genes was shown by microarray and qPCR analyses to increase in the migrating epithelium of wounded corneas. Many of these enzymes were converted into their enzymatically active form as wound closure proceeded. In addition, expression of MMPs by human corneal epithelial cells (HCECs) was affected both by the stromal fibroblasts and the collagen-enriched ECM they produce. Most of all, results from mass spectrometry analyses provided evidence that a fully stratified epithelium is required for proper synthesis and organization of the ECM on which the epithelial cells adhere. In conclusion, and because of the many characteristics it shares with the native cornea, this human two layers corneal substitute may prove particularly useful to decipher the mechanistic details of corneal wound healing.
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| Overall design |
Primary cultures of human corneal epithelial cells cultivated on BSA (number of replicates: 7), Collagen type I (number of replicates: 2), Collagen type IV (number of replicates: 2), Fibronectin (number of replicates: 2), Tenascin C (number of replicates: 2) and Laminin (number of replicates: 2) matrix. Central, internal and external ring of wounded Tissue-engineered human cornea.
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| Contributor(s) |
Couture C, Zaniolo K, Carrier P, Lake J, Patenaude J, Germain L, Guérin SL |
| Citation(s) |
26686051, 34830308 |
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| Submission date |
Nov 24, 2015 |
| Last update date |
Dec 03, 2021 |
| Contact name |
Karine Zaniolo |
| E-mail(s) |
karine.zaniolo@gmail.com
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| Organization name |
Centre de recherche du CHU de Québec
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| Department |
CUO-Recherche
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| Lab |
Sylvain Guérin
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| Street address |
1050 Chemin Sainte-Foy
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| City |
Québec |
| State/province |
Québec |
| ZIP/Postal code |
G1S 4L8 |
| Country |
Canada |
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| Platforms (1) |
| GPL13607 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version) |
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| Samples (27)
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| GSM1953464 |
Primary_human_corneal_epithelial_cells_BSA_repl1 |
| GSM1953465 |
Primary_human_corneal_epithelial_cells_BSA_repl2 |
| GSM1953466 |
Primary_human_corneal_epithelial_cells_BSA_repl3 |
| GSM1953467 |
Primary_human_corneal_epithelial_cells_BSA_repl4 |
| GSM1953468 |
Primary_human_corneal_epithelial_cells_BSA_repl5 |
| GSM1953469 |
Primary_human_corneal_epithelial_cells_BSA_repl6 |
| GSM1953471 |
Primary_human_corneal_epithelial_cells_Collagen Type I_repl1 |
| GSM1953472 |
Primary_human_corneal_epithelial_cells_Collagen Type I_repl2 |
| GSM1953473 |
Primary_human_corneal_epithelial_cells_Collagen Type IV_repl1 |
| GSM1953474 |
Primary_human_corneal_epithelial_cells_Collagen Type IV_repl2 |
| GSM1953475 |
Primary_human_corneal_epithelial_cells_Fibronectin_repl1 |
| GSM1953476 |
Primary_human_corneal_epithelial_cells_Fibronectin_repl2 |
| GSM1953477 |
Primary_human_corneal_epithelial_cells_Tenascin_C_repl1 |
| GSM1953478 |
Primary_human_corneal_epithelial_cells_Tenascin_C_repl2 |
| GSM1953479 |
Primary_human_corneal_epithelial_cells_Laminin_repl1 |
| GSM1953480 |
Primary_human_corneal_epithelial_cells_Laminin_repl2 |
| GSM1953481 |
Central_ring_of_wounded_Tissue-engineered_human_replicate_1 |
| GSM1953482 |
Central_ring_of_wounded_Tissue-engineered_human_replicate_2 |
| GSM1953483 |
Central_ring_of_wounded_Tissue-engineered_human_replicate_3 |
| GSM1953484 |
Internal_ring_of_wounded_Tissue-engineered_human_replicate_1 |
| GSM1953485 |
Internal_ring_of_wounded_Tissue-engineered_human_replicate_2 |
| GSM1953486 |
Internal_ring_of_wounded_Tissue-engineered_human_replicate_3 |
| GSM1953487 |
External_ring_of_wounded_Tissue-engineered_human_replicate_1 |
| GSM1953488 |
External_ring_of_wounded_Tissue-engineered_human_replicate_2 |
| GSM1953489 |
External_ring_of_wounded_Tissue-engineered_human_replicate_3 |
| GSM1953490 |
Control_unwounded_of_Tissue-engineered_human_cornea_replicate_1 |
| GSM1953491 |
Control_unwounded_of_Tissue-engineered_human_cornea_replicate_2 |
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| Relations |
| BioProject |
PRJNA304092 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE75336_RAW.tar |
337.9 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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