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Series GSE75336 Query DataSets for GSE75336
Status Public on Nov 25, 2015
Title The tissue-engineered human cornea as a model to study expression of matrix metalloproteinases during corneal wound healing
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Corneal injuries remain a major cause of consultation in the ophthalmology clinics worldwide. Repair of corneal wounds is a complex mechanism that involves cell death, migration, proliferation, differentiation, and extracellular matrix (ECM) remodeling. In the present study, we used a tissue-engineered, two-layers (epithelium and stroma) human cornea as a biomaterial to study both the cellular and molecular mechanisms of wound healing. Gene profiling on microarrays revealed important alterations in the pattern of genes expressed by tissue-engineered corneas in response to wound healing. Expression of many MMPs-encoding genes was shown by microarray and qPCR analyses to increase in the migrating epithelium of wounded corneas. Many of these enzymes were converted into their enzymatically active form as wound closure proceeded. In addition, expression of MMPs by human corneal epithelial cells (HCECs) was affected both by the stromal fibroblasts and the collagen-enriched ECM they produce. Most of all, results from mass spectrometry analyses provided evidence that a fully stratified epithelium is required for proper synthesis and organization of the ECM on which the epithelial cells adhere. In conclusion, and because of the many characteristics it shares with the native cornea, this human two layers corneal substitute may prove particularly useful to decipher the mechanistic details of corneal wound healing.
Overall design Primary cultures of human corneal epithelial cells cultivated on BSA (number of replicates: 7), Collagen type I (number of replicates: 2), Collagen type IV (number of replicates: 2), Fibronectin (number of replicates: 2), Tenascin C (number of replicates: 2) and Laminin (number of replicates: 2) matrix. Central, internal and external ring of wounded Tissue-engineered human cornea.
Contributor(s) Couture C, Zaniolo K, Carrier P, Lake J, Patenaude J, Germain L, Guérin SL
Citation(s) 26686051, 34830308
Submission date Nov 24, 2015
Last update date Dec 03, 2021
Contact name Karine Zaniolo
Organization name Centre de recherche du CHU de Québec
Department CUO-Recherche
Lab Sylvain Guérin
Street address 1050 Chemin Sainte-Foy
City Québec
State/province Québec
ZIP/Postal code G1S 4L8
Country Canada
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (27)
GSM1953464 Primary_human_corneal_epithelial_cells_BSA_repl1
GSM1953465 Primary_human_corneal_epithelial_cells_BSA_repl2
GSM1953466 Primary_human_corneal_epithelial_cells_BSA_repl3
BioProject PRJNA304092

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE75336_RAW.tar 337.9 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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