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| Status |
Public on Jan 07, 2016 |
| Title |
Translation State Array Assay for C elegans IFE-1-dependent mRNAs |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by array
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| Summary |
Relative polysomal loading changes for wild type (N2) versus ife-1(bn127) C. elegans strains
Strain ife-1(bn127) is null for the gene encoding one of five eIF4E isoforms in the worm, IFE-1. Only this isoform associates with P granules.
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| Overall design |
Resolution of translating and non-translating mRNAs from WT and mutant worms on 10-45% sucrose gradients. Comparision was by ratios of polysomal content.
Please note that each sample data table contains raw signal directly imported from the Affymetrix files and the ratio of signals, P/(NP+P), and fold-changes were further calculated. The TSAA_overview.xls contains more datails about the TSAA procedure and 'complete_data.xlsx' contains complete TSAA data output.
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| Contributor(s) |
Keiper BD, Friday AJ, Henderson MA, Morrison JK, Hoffman JL |
| Citation(s) |
26542024 |
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| Submission date |
Oct 28, 2015 |
| Last update date |
Jul 06, 2016 |
| Contact name |
Brett D Keiper |
| E-mail(s) |
keiperb@ecu.edu
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| Phone |
252-744-2656
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| Organization name |
Brody School of Med at ECU
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| Department |
Biochem and Mol Biol
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| Street address |
600 Moye Blvd
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| City |
Greenville |
| State/province |
NC |
| ZIP/Postal code |
27834 |
| Country |
USA |
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| Platforms (1) |
| GPL200 |
[Celegans] Affymetrix C. elegans Genome Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA300453 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE74459_RAW.tar |
26.7 Mb |
(http)(custom) |
TAR (of CEL) |
| GSE74459_TSAA_overview.xls.gz |
6.4 Kb |
(ftp)(http) |
XLS |
| GSE74459_complete_data.xlsx |
8.6 Mb |
(ftp)(http) |
XLSX |
| Processed data are available on Series record |
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