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| Status |
Public on Apr 10, 2007 |
| Title |
Laser capture-microarray analysis of Lim1 mutant kidney development. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The Lim1 gene has essential functions during several stages of kidney development. In particular, a tissue specific knockout in the early metanephric mesenchyme results in the formation of the earliest nephron precursor, the renal vesicle, but failure of this structure to progress to the next stage, the comma shaped body. To better understand the molecular nature of this developmental arrest we used a laser capture microdissection-microarray strategy to examine the perturbed gene expression pattern of the mutant renal vesicles. Among the genes found differently expressed were Chrdl2, an inhibitor of BMP signaling, the pro-apoptotic factor Bmf, as well as myob5, an atypical myosin which modulates chemokine and transferring signaling, and pdgfr1, which is important in epithelial folding. Of particular interest, the microarray data indicated that the Dkk1 gene, which encodes an inhibitor of Wnt signaling, was downregulated nine fold in mutants. This was confirmed by in situ hybridizations. It is interesting to note that Lim1 and Dkk1 mutant mice have striking similarities in phenotype. These results suggest that the Dkk1 gene might be a key downstream effector of Lim1 function.
Keywords: Lim1 mutant, kidney development, gene expression profile comparison
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| Overall design |
We compared gene expression profiles of wild type and mutant Lim1 renal vesicles, which were isolated from E12.5 kidneys by laser capture microdissection. Nine biological independent samples were examined, with four mutant and five wild type. Each sample included approximately 30-50 renal vesicles. Detailed protocols are described at GUDMAP.org
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| Contributor(s) |
Potter S, Hartman HA, Kwan KM, Behringer RR, Patterson LT |
| Citation(s) |
17610272 |
| Submission date |
Mar 13, 2007 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Steven Potter |
| E-mail(s) |
steve.potter@cchmc.org
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| Organization name |
Children's hospital Medical Center
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| Department |
Developmental Biology
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| Lab |
Potter
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| Street address |
3333 Burnet Ave.
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| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA98013 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE7257_RAW.tar |
78.6 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data provided as supplementary file |
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