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| Status |
Public on Apr 29, 2015 |
| Title |
Differential gene expression profiling by LSD1 inhibition using novel LSD1 inhibitors (NCD25 and NCD38) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
LSD1 is a demethylase of histone modification H3k4me1 and H3K4me2. We have developed novel LSD1 inhibitors (NCD25 and NCD38) and found that they are effective to myelodysplastic syndromes and leukemia cells. To understand what mechanisms are affected by these compounds, we employed gene expression profiling analyses. Gene expression profiling data were obtained from HEL, MDS-L, or CMK11-5 cells treated with DMSO (control), NCD25, or NCD38 and compared each other. Expression of eleven transcriptional factors (GFI1, CEBPA, SPI1, MNDA, TAL1, GATA1, NFE2, RXRA, HOXA9, GATA2, and PBX1) was reconfirmed by q-PCR with the same samples.
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| Overall design |
Gene expression of leukemia cells was measured after 48 hours incubation with or without LSD1 inhibitors. Five independent experiments were performed using 3 cell lines (HEL, MDS-L and CMK11-5) and 2 drugs (NCD38 and NCD25).
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| Contributor(s) |
Sugino N, Kawahara M |
| Citation missing |
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| Submission date |
Apr 28, 2015 |
| Last update date |
Jan 09, 2018 |
| Contact name |
Masahiro Kawahara |
| E-mail(s) |
machakm6@kuhp.kyoto-u.ac.jp
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| Phone |
+81757514964
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| Organization name |
Kyoto University
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| Department |
Hematology Oncology
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| Lab |
leukemia research
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| Street address |
54 Shogoin-Kawahara-cho, Sakyo-ku,
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| City |
Kyoto |
| State/province |
State... |
| ZIP/Postal code |
6068507 |
| Country |
Japan |
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| Platforms (1) |
| GPL17077 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA282493 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE68348_RAW.tar |
73.4 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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