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Series GSE68348 Query DataSets for GSE68348
Status Public on Apr 29, 2015
Title Differential gene expression profiling by LSD1 inhibition using novel LSD1 inhibitors (NCD25 and NCD38)
Organism Homo sapiens
Experiment type Expression profiling by array
Summary LSD1 is a demethylase of histone modification H3k4me1 and H3K4me2. We have developed novel LSD1 inhibitors (NCD25 and NCD38) and found that they are effective to myelodysplastic syndromes and leukemia cells. To understand what mechanisms are affected by these compounds, we employed gene expression profiling analyses. Gene expression profiling data were obtained from HEL, MDS-L, or CMK11-5 cells treated with DMSO (control), NCD25, or NCD38 and compared each other. Expression of eleven transcriptional factors (GFI1, CEBPA, SPI1, MNDA, TAL1, GATA1, NFE2, RXRA, HOXA9, GATA2, and PBX1) was reconfirmed by q-PCR with the same samples.
Overall design Gene expression of leukemia cells was measured after 48 hours incubation with or without LSD1 inhibitors. Five independent experiments were performed using 3 cell lines (HEL, MDS-L and CMK11-5) and 2 drugs (NCD38 and NCD25).
Contributor(s) Sugino N, Kawahara M
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Submission date Apr 28, 2015
Last update date Jan 09, 2018
Contact name Masahiro Kawahara
Phone +81757514964
Organization name Kyoto University
Department Hematology Oncology
Lab leukemia research
Street address 54 Shogoin-Kawahara-cho, Sakyo-ku,
City Kyoto
State/province State...
ZIP/Postal code 6068507
Country Japan
Platforms (1)
GPL17077 Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version)
Samples (24)
GSM1668911 HEL_DMSO_48h_rep1
GSM1668912 HEL_DMSO_48h_rep2
GSM1668913 HEL_DMSO_48h_rep3
BioProject PRJNA282493

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE68348_RAW.tar 73.4 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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