NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE65419 Query DataSets for GSE65419
Status Public on Jan 30, 2015
Title Transcriptional profiling of drug-tolerant cancer cell subpopulations
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Cancer relapse occurs even after curative treatment, suggesting the existence of undetectable cancer cell subpopulations and their drug-tolerant potential. We found that the number of drug-tolerant colonies (DTCs) was significantly suppressed by an RNA polymerase II (RNAPII) inhibitor, alpha-amanitin (alpha-AMA). To identify potential molecular target of alpha-AMA, we performed transcriptional profiling by Agilent-028004 SurePrint G3 Human GE 8x60K Microarray using untreated colonies and DTCs derived from MCF7. Among the top 2.5% of specifically induced genes in DTCs, we focused on TAF15 gene because its gene product binds RNAPII. A subsequent colony formation assay revealed that TAF15 knockdown suppressed the emergence of both DTCs and untreated colonies, suggesting that TAF15 is a crucial target of alpha-AMA in the context of DTC suppression.
 
Overall design Total RNA was extracted from two biological replicates of bulk untreated MCF7 colonies and MCF7 DTCs emerged in the presence of 0.8 ┬ÁM CIS. For identification of the TAF15 gene, gene ontology analysis was performed using Database for Annotation, Visualization and Integrated Discovery (DAVID) software tools (http://david.abcc.ncifcrf.gov/).
 
Contributor(s) Kume K, Nishizuka SS
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Jan 29, 2015
Last update date Nov 27, 2018
Contact name Kohei Kume
E-mail(s) kkume@iwate-med.ac.jp
Organization name Iwate Medical University
Street address 19-1 Uchimaru
City Morioka
ZIP/Postal code 0808505
Country Japan
 
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (4)
GSM1595835 MCF7colonies_untreated_1
GSM1595836 MCF7colonies_untreated_2
GSM1595837 MCF7colonies_cisplatin_1
Relations
BioProject PRJNA273955

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE65419_RAW.tar 54.4 Mb (http)(custom) TAR (of TXT)
GSE65419_normalized_data_with_probe_name.txt.gz 1.6 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap