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| Status |
Public on Jan 21, 2015 |
| Title |
FASCIATED EAR4 encodes a bZIP transcription factor that controls shoot meristem size in maize |
| Organism |
Zea mays |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
fasciated ear4 (fea4) is a semi-dwarfed mutant with fasciated ears and tassels, and greatly enlarged vegetative and inflorescence meristems. Chromatin Immunoprecipitation-sequencing (ChIP-seq) and expression profiling by RNA-seq suggest that fea4 is required to regulate the auxin response and leaf differentiation programs in the periphery of the meristem, suggesting a new mechanism of meristem size regulation that is spatially and mechanistically distinct from the CLV-WUS model.
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| Overall design |
To gain insight into transcriptional regulation by FEA4, we used RNA-seq to profile genome-wide expression changes in developing ear primordia of fea4 loss-of-function mutants compared to fea4/+ wild-type (wt) siblings. To map genome-wide occupancy of FEA4 and define its putative transcriptional targets, we performed ChIP-seq using the pFEA4-YFP::FEA4 transgenic lines.
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| Contributor(s) |
Eveland AL, Pautler M, Jackson D |
| Citation(s) |
25616871 |
| |
| Submission date |
Oct 01, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Andrea L Eveland |
| E-mail(s) |
aeveland@danforthcenter.org
|
| Organization name |
Donald Danforth Plant Science Center
|
| Street address |
975 N. Warson Road
|
| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63132 |
| Country |
USA |
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| Platforms (2) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA262781 |
| SRA |
SRP048564 |
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