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| Status |
Public on Feb 12, 2015 |
| Title |
A unique chromatin complex occupies young alpha-satellite arrays of human centromeres |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Using high-resolution chromatin immunoprecipitation (ChIP) of centromere components and clustering of sequence data we find that specific dimeric alpha-satellite units shared by multiple individuals dominate functional human centromeres.
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| Overall design |
We have analyzed the chromatin landscape of the human genome using paired-end MNase-seq
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| Contributor(s) |
Henikoff JG, Thakur J, Henikoff S |
| Citation(s) |
25927077 |
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| Submission date |
Aug 29, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Jorja Henikoff |
| E-mail(s) |
jorja@fhcrc.org
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| Phone |
206-667-4850
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| Organization name |
Fred Hutchinson Cancer Research Center
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| Department |
Basic Sciences
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| Lab |
Henikoff
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| Street address |
1100 Fairview AV N, A1-162
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| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109-1024 |
| Country |
USA |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (19)
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| GSM1494427 |
HuRef_Input_2B_(20140605_2,5) |
| GSM1494428 |
HuRef_CenpA_1A_(20140605_1,2) |
| GSM1494429 |
HuRef_CenpA_1B_(20140605_2,2) |
| GSM1494430 |
HuRef_CenpA_2A_(20140605_1,8) |
| GSM1494431 |
HuRef_CenpA_2B_(20140605_2,8) |
| GSM1494432 |
HuRef_CenpC_1A_(20140605_1,4) |
| GSM1494433 |
HuRef_CenpC_1B_(20140605_2,4) |
| GSM1494434 |
HuRef_CenpC_2A_(20140605_1,9) |
| GSM1494435 |
HuRef_CenpC_2B_(20140605_2,9) |
| GSM1494436 |
HuRef_H3_A_(20140605_1,10) |
| GSM1494437 |
HuRef_H3_B_(20140605_2,10) |
| GSM1494438 |
HuRef_M2_A_(20140605_1,11) |
| GSM1494439 |
HuRef_M2_B_(20140605_2,11) |
| GSM1494440 |
HeLa_Input_(20140605_1,6) |
| GSM1494441 |
HeLa_CenpA_(20140605_2,7) |
| GSM1494442 |
HeLa_CenpC_(20140605_2,12) |
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| Relations |
| BioProject |
PRJNA259887 |
| SRA |
SRP045908 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data not provided for this record |
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