|
Status |
Public on Feb 12, 2015 |
Title |
HeLa_Input_(20140605_1,6) |
Sample type |
SRA |
|
|
Source name |
HeLa input
|
Organism |
Homo sapiens |
Characteristics |
cell: nuclei cell type: Epithelial carcinoma cell line: HeLa chip antibody: None
|
Extracted molecule |
genomic DNA |
Extraction protocol |
The HeLa cell line was grown in DMEM media using strandard protocols. Input DNA was prepared from cross-linked cells as described previously (PMID:24737864).
|
|
|
Library strategy |
MNase-Seq |
Library source |
genomic |
Library selection |
MNase |
Instrument model |
Illumina HiSeq 2500 |
|
|
Description |
HeLa cross-linked (X-ChIP) input
|
Data processing |
Paired reads were merged using SeqPrep (https://github.com/jstjohn/SeqPrep) with parameters -q 30 (quality) and -L 25 (minimum merged pair length). SeqPrep also removed adaptors and low-quality reads.
|
|
|
Submission date |
Aug 29, 2014 |
Last update date |
May 15, 2019 |
Contact name |
Jorja Henikoff |
E-mail(s) |
jorja@fhcrc.org
|
Phone |
206-667-4850
|
Organization name |
Fred Hutchinson Cancer Research Center
|
Department |
Basic Sciences
|
Lab |
Henikoff
|
Street address |
1100 Fairview AV N, A1-162
|
City |
Seattle |
State/province |
WA |
ZIP/Postal code |
98109-1024 |
Country |
USA |
|
|
Platform ID |
GPL16791 |
Series (1) |
GSE60951 |
A unique chromatin complex occupies young alpha-satellite arrays of human centromeres |
|
Relations |
BioSample |
SAMN03013866 |
SRA |
SRX690218 |