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| Status |
Public on Mar 02, 2018 |
| Title |
Gene expression in human bone marrow Mesenchymal Stem cell line (hMSC-TERTs) during proliferation, quiescence (G0-arrest) and re-activation. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
A microarray study using human Mesenchymal Stem cell line (hMSC-TERTs), was designed in order to detect the differences in global gene expression during proliferation, quiescence (G0) and reactivation post quiescence for 1 hr (R1), 3 hrs (R3) and 24 hrs (R24). Proliferating and re-activated cells were harvested at 70-75% confluency. To induce quiescence, MSCs were cultured in suspension in methyl cellulose in presence of full serum complement. The raw data from gene arrays were normalized and the five samples were compared to each other in order to detect differences in gene expression between the samples.
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| Overall design |
Asynchronously proliferating hMSCs-TERTs was cultured in two different culture conditions, in adherent condition (proliferating) and in non-adherent suspension condition (quiescent). Gene expression profiling of proliferating, quiescent and re-activated hMSCs was performed using agilent arrays. All the samples were taken in triplicates. Thus the numeber of samples for microarray analysis was 15.
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| Contributor(s) |
Rumman M, Majumder A, Harkness L, Venugopal B, Vinay BR, Pillai MS, Kassem M, Dhawan J |
| Citation(s) |
29803144 |
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| Submission date |
Aug 21, 2014 |
| Last update date |
Jul 13, 2018 |
| Contact name |
Genotypic technology |
| E-mail(s) |
genomics@genotypic.co.in
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| Organization name |
Genotypic Technology
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| Street address |
259, Apoorva 4th cross,80 feet Road,RMV 2ND STAGE
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| City |
Bangalore |
| State/province |
Karnataka |
| ZIP/Postal code |
560094 |
| Country |
India |
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| Platforms (1) |
| GPL17077 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version) |
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| Samples (15)
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| GSM1483636 |
Quiescent hMSC_TERTs,rep 1 |
| GSM1483637 |
Quiescent hMSC_TERTs,rep 2 |
| GSM1483638 |
Quiescent hMSC_TERTs,rep 3 |
| GSM1483639 |
hMSC_TERTs reactivated after G0 for 1hr (R1), rep 1 |
| GSM1483640 |
hMSC_TERTs reactivated after G0 for 1hr (R1), rep 2 |
| GSM1483641 |
hMSC_TERTs reactivated after G0 for 1hr (R1), rep 3 |
| GSM1483642 |
hMSC_TERTs reactivated after G0 for 3hrs (R3), rep 1 |
| GSM1483643 |
hMSC_TERTs reactivated after G0 for 3hrs (R3), rep 2 |
| GSM1483644 |
hMSC_TERTs reactivated after G0 for 3hrs (R3), rep 3 |
| GSM1483645 |
hMSC_TERTs reactivated after G0 for 24hrs (R24), rep 1 |
| GSM1483646 |
hMSC_TERTs reactivated after G0 for 24hrs (R24), rep 2 |
| GSM1483647 |
hMSC_TERTs reactivated after G0 for 24hrs (R24), rep 3 |
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| Relations |
| BioProject |
PRJNA258644 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE60608_RAW.tar |
184.8 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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