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Series GSE60608 Query DataSets for GSE60608
Status Public on Mar 02, 2018
Title Gene expression in human bone marrow Mesenchymal Stem cell line (hMSC-TERTs) during proliferation, quiescence (G0-arrest) and re-activation.
Organism Homo sapiens
Experiment type Expression profiling by array
Summary A microarray study using human Mesenchymal Stem cell line (hMSC-TERTs), was designed in order to detect the differences in global gene expression during proliferation, quiescence (G0) and reactivation post quiescence for 1 hr (R1), 3 hrs (R3) and 24 hrs (R24). Proliferating and re-activated cells were harvested at 70-75% confluency. To induce quiescence, MSCs were cultured in suspension in methyl cellulose in presence of full serum complement. The raw data from gene arrays were normalized and the five samples were compared to each other in order to detect differences in gene expression between the samples.
Overall design Asynchronously proliferating hMSCs-TERTs was cultured in two different culture conditions, in adherent condition (proliferating) and in non-adherent suspension condition (quiescent). Gene expression profiling of proliferating, quiescent and re-activated hMSCs was performed using agilent arrays. All the samples were taken in triplicates. Thus the numeber of samples for microarray analysis was 15.
Contributor(s) Rumman M, Majumder A, Harkness L, Venugopal B, Vinay BR, Pillai MS, Kassem M, Dhawan J
Citation(s) 29803144
Submission date Aug 21, 2014
Last update date Jul 13, 2018
Contact name Genotypic technology
Organization name Genotypic Technology
Street address 259, Apoorva 4th cross,80 feet Road,RMV 2ND STAGE
City Bangalore
State/province Karnataka
ZIP/Postal code 560094
Country India
Platforms (1)
GPL17077 Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version)
Samples (15)
GSM1483633 Proliferating hMSC_TERTs, rep 1
GSM1483634 Proliferating hMSC_TERTs, rep 2
GSM1483635 Proliferating hMSC_TERTs, rep 3
BioProject PRJNA258644

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Supplementary file Size Download File type/resource
GSE60608_RAW.tar 184.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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