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Series GSE59703 Query DataSets for GSE59703
Status Public on Dec 12, 2014
Title Browning of human adipocytes requires KLF11 and reprogramming of PPARγ super-enhancers
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary Here we have characterized the transcriptional processes underlying the formation of human brown in white (i.e. brite) adipocytes using a genome-wide approach. We show that the browning process is associated with reprogramming of peroxisome proliferator-activated receptor γ (PPARγ) binding to form brite adipocyte-selective PPARγ super-enhancers that appear to play a key role in activation of brite adipocyte-selective genes. We identify the KLF11 gene based on its association with a PPARγ super-enhancer and show that KLF11 is a novel browning factor directly induced by rosiglitazone and required for the activation of brite adipocyte-selective gene program by rosiglitazone.
Overall design Genome-wide profiling of Dnase I hypersenstive (DHS) sites, epigenomic marks, transcription factor and co-factor binding, and gene expression in hMADS white and brite adipocytes
Contributor(s) Loft A, Mandrup S
Citation(s) 25504365
Submission date Jul 23, 2014
Last update date Feb 21, 2024
Contact name Anne Loft
Organization name University of Southern Denmark
Department Biochemistry & Molecular Biology
Street address Campusvej 55
City Odense M
ZIP/Postal code 5230
Country Denmark
Platforms (2)
GPL10999 Illumina Genome Analyzer IIx (Homo sapiens)
GPL18460 Illumina HiSeq 1500 (Homo sapiens)
Samples (36)
GSM1443801 DHS hMADS white, day 19
GSM1443802 DHS hMADS brite, day 19
GSM1443803 CBP hMADS white, day 19
BioProject PRJNA255992
SRA SRP044756

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE59703_AllPPARgSuperenhancers.bed.gz 16.2 Kb (ftp)(http) BED
GSE59703_BriteselectivePPARgPeaks.bed.gz 31.8 Kb (ftp)(http) BED
GSE59703_BriteselectivePPARgSuperenhancers.bed.gz 4.2 Kb (ftp)(http) BED
GSE59703_Briteselectivegenes.txt.gz 148.0 Kb (ftp)(http) TXT
GSE59703_ConstantPPARgPeaks.bed.gz 34.4 Kb (ftp)(http) BED
GSE59703_ConstantPPARgSuperenhancers.bed.gz 2.0 Kb (ftp)(http) BED
GSE59703_Constitutivegenes.txt.gz 82.8 Kb (ftp)(http) TXT
GSE59703_KLF11KDnormalizedlog2readsperkilobase.txt.gz 341.6 Kb (ftp)(http) TXT
GSE59703_KLF11regulatedgenes.txt.gz 126.6 Kb (ftp)(http) TXT
GSE59703_Normalizedlog2readsperkilobase.txt.gz 1.8 Mb (ftp)(http) TXT
GSE59703_RAW.tar 823.8 Mb (http)(custom) TAR (of BEDGRAPH)
GSE59703_WhiteselectivePPARgPeaks.bed.gz 31.8 Kb (ftp)(http) BED
GSE59703_WhiteselectivePPARgSuperenhancers.bed.gz 4.0 Kb (ftp)(http) BED
GSE59703_Whiteselectivegenes.txt.gz 100.7 Kb (ftp)(http) TXT
GSE59703_hMADSDHSPeakfile.bed.gz 373.8 Kb (ftp)(http) BED
GSE59703_hMADSKLF11Peakfile.bed.gz 494.8 Kb (ftp)(http) BED
GSE59703_hMADSPPARgPeakfile.bed.gz 801.8 Kb (ftp)(http) BED
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Raw data are available in SRA
Processed data provided as supplementary file

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