|
| Status |
Public on Sep 01, 2014 |
| Title |
Identification of transcripts regulated by CEBPA in K562 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
To investigate the effect of CEBPA on the expression of mRNAs and long non-coding RNAs ( lncRNAs), we utilized the K562 AML cell line carrying a stable and Tet-on inducible CEBPA allele. K562 cells lack endogenous CEBPA and restoration of its expression induces proliferation arrest and granulocytic differentiation .
|
| |
|
| Overall design |
Based on the expression of known CEBPA transcriptional targets, we selected RNA extracted from 48 hours of induction (CEBPA) together with RNA extracted from control-induced cells (CTR). 4 biological replicates for each sample have been utilized.
|
| |
|
| Contributor(s) |
Hughes J, Salvatori B, Fatica A |
| Citation(s) |
25252716 |
| Submission date |
Jun 23, 2014 |
| Last update date |
Jan 09, 2018 |
| Contact name |
Alessandro Fatica |
| E-mail(s) |
alessandro.fatica@uniroma1.it
|
| Organization name |
Sapienza University of Rome
|
| Department |
Biology and Biotechnology "C. Darwin"
|
| Street address |
P.le Aldo Moro 5
|
| City |
Rome |
| ZIP/Postal code |
00185 |
| Country |
Italy |
| |
|
| Platforms (1) |
| GPL17077 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA253317 |
Less...
More...