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Series GSE56989 Query DataSets for GSE56989
Status Public on Nov 30, 2014
Title Genome-wide identification of HIF-1 and HIF-2 binding sites in hypoxic human macrophages alternatively activated by IL-10
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Primary human macrophages with a HIF-1alpha or HIF-2alpha knockdown were pretreated with IL-10 for 16h and afterwards for 4h additionaly under hypoxi (1% O2), RNA was isolated usind the Qiagen RNAeasy Kit and cDNA synthesis wos done using Ambion WT Expression Kit. Expression was compared to si control under control conditions.
 
Overall design Samples from 3 donors treated with si control, si HIF-1α or si HIF-2α were compared with each other
 
Contributor(s) Dehne N, Döring C
Citation(s) 25450522
Submission date Apr 23, 2014
Last update date Jul 26, 2018
Contact name Nathalie Dehne
E-mail(s) dehne@biochem.uni-frankfurt.de
Organization name Goethe-University Frankfurt
Department Faculty of Medicine, Institute of Biochemestry I
Street address Theodor-Stern-Kai 7
City Frankfurt
ZIP/Postal code 60590
Country Germany
 
Platforms (1)
GPL6244 [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version]
Samples (36)
GSM1372675 human macrophages (si control) 16h without treatment, 4h 21% oxygen [1N1]
GSM1372676 human macrophages (si control) 16h without treatment, 4h 21% oxygen [1N2]
GSM1372677 human macrophages (si control) 16h without treatment, 4h 21% oxygen [1N3]
Relations
BioProject PRJNA245176

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE56989_RAW.tar 142.9 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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