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Series GSE55774 Query DataSets for GSE55774
Status Public on Aug 12, 2014
Title Stress-induced signaling to the nucleosome in serum deprived mouse fibroblasts.
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We have employed chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) to analyze changes in chromatin architecture as well as the occupancy of two RNA Polymerase II (RNAPII) isoforms, initiation-competent (RNAPIIS5ph) as well as elongation-competent (RNAPIIS2ph) upon stress induction. We used resting mouse Swiss 3T3 fibroblasts, either untreated (control) or treated with anisomycin to induce the p38/MAP kinase pathway.
Overall design Serum starved (72 h 0.2% FCS) mouse 3T3 cells were treated with anisomycin (188.5 nM) for 1 h (in duplicates). Untreated, serum-starved cells were used as a control. Isolated chromatin was subjected to immunoprecipitation with the following antibodies: a-H3S28ph, a-H3K9ac, a-H3K4me3, a-RNAPIIS5ph and a-RNAPIIS2ph. Resulting DNA was sequenced using Illumina platforms.
Contributor(s) Sawicka A, Seiser C
Citation(s) 25135956
Submission date Mar 11, 2014
Last update date May 15, 2019
Contact name Anna Sawicka
Organization name Max Planck Institure for Biophysical Chemistry
Department Dep. of Molecular Biology
Lab Cramer
Street address Am Fassberg 11
City Goettingen
ZIP/Postal code 37077
Country Germany
Platforms (2)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (22)
GSM1345927 input_control_untreated
GSM1345928 input_1hA
GSM1345929 H3S28ph_control_untreated_replicate1
This SubSeries is part of SuperSeries:
GSE55784 Genome-wide analysis of stress response in mouse fibroblasts
BioProject PRJNA240993
SRA SRP039955

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Supplementary file Size Download File type/resource
GSE55774_RAW.tar 2.9 Gb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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