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| Status |
Public on Jul 08, 2016 |
| Title |
Genomic features shaping the landscape of meiotic double-strand break hotspots in maize [ChIP-Seq] |
| Organism |
Zea mays |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
To generate the double-strand break (DSB) hotspot map in maize, we used a chromatin immunoprecipitation (ChIP) approach, in which chromatin from flowers containing zygotene meiocytes was enriched in fragments associated with RAD51.
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| Overall design |
To identify sites occupied by RAD51, we compared the genomic distribution of RAD51 ChIP-seq reads to (i) Illumina-sequenced meiotic input chromatin that was not subjected to ChIP, (ii) ChIP products generated by using pre-immune IgG, instead of the anti-RAD51 antibody, on meiotic chromatin, and (iii) ChIP products generated using the anti-RAD51 antibody on leaf tissue chromatin. RAD51 ChIP-seq was also performed on the meiotic chromatin of a POOR HOMOLOGOUS SYNAPSIS1 (phs1) mutant.
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| |
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| Contributor(s) |
Pawlowski WP, He Y |
| Citation(s) |
29087335 |
| |
| Submission date |
Mar 07, 2014 |
| Last update date |
Jun 13, 2019 |
| Contact name |
Wojciech Pawlowski |
| E-mail(s) |
mw729@cornell.edu
|
| Organization name |
Cornell University
|
| Department |
Plant Breeding and Genetics
|
| Street address |
Bradfield Hall, Room 403
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| City |
Ithaca |
| State/province |
NY |
| ZIP/Postal code |
14853 |
| Country |
USA |
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| Platforms (1) |
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| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE84369 |
Genomic features shaping the landscape of meiotic double-strand break hotspots in maize |
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| Relations |
| BioProject |
PRJNA240574 |
| SRA |
SRP039553 |
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