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| Status |
Public on May 20, 2015 |
| Title |
An analysis of genomic structural alteration in three human ES cell lines. |
| Organism |
Homo sapiens |
| Experiment type |
SNP genotyping by SNP array
|
| Summary |
To elucidate frequencies of genomic structural alterations, we performed an analysis using a SNP genotyping array (Illumina HumanCytoSNP-12 v2.1 DNA Analysis BeadChip Kit) for three human ES cell lines, namely, SEES-1, SEES-2, and SEES-3. Samples were collected after 4 to 60 passages to detect structural mutations occurred during the cell cultivation processes. Our results suggested a higher genomic stability of the ES cells compared to some well-known normal cell lines.
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| Overall design |
Several samples were collected after 4 to 60 passages and subjected to the SNP genotyping array. We searched for structural mutations occurred during the culture of the ES cells.
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| Contributor(s) |
Akutsu H, Nakabayashi K, Okamura K, Hata K, Umezawa A |
| Citation(s) |
24970375 |
| |
| Submission date |
Jan 30, 2014 |
| Last update date |
Dec 18, 2017 |
| Contact name |
Akihiro Umezawa |
| E-mail(s) |
umezawa@1985.jukuin.keio.ac.jp
|
| Phone |
81-3-5494-7047
|
| Organization name |
National Center for Child Health and Development
|
| Department |
Center for Regenerative Medicine
|
| Street address |
2-10-1 Okura
|
| City |
Setagaya-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
157-8535 |
| Country |
Japan |
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| Platforms (1) |
| GPL13829 |
Illumina HumanCytoSNP-12 v2.1 BeadChip |
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| Samples (11)
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| Relations |
| BioProject |
PRJNA237441 |
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