|
| Status |
Public on May 20, 2015 |
| Title |
SEES3 at passage 25 |
| Sample type |
genomic |
| |
|
| Source name |
human embryonic stem cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: SEES3
passage: 25
|
| Growth protocol |
The SEES lines were routinely cultured onto a feeder layer of freshly plated gamma-irradiated MEFs in the human ES cell culture media. The media consisted of KnockoutTM-Dulbecco’s modified Eagle’s medium supplemented with 20% KnockoutTM-Serum Replacement, 2 mM Glutamax-I, 0.1 mM non-essential amino acids, 50 U/ml penicillin-50 μg/ml streptomycin (Pen-Strep), 0.055 mM beta-mercaptoethanol, and recombinant human full-length bFGF.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA extracted from cells using QIAamp DNA Mini Kit (Qiagen).
|
| Label |
C-Bio and A-DNP
|
| Label protocol |
Illumina's standard protocol
|
| |
|
| Hybridization protocol |
DNA was amplified, fragmented and hybridized to each BeadChip arrays according to the manufacturer's instructions.
|
| Scan protocol |
The BeadChip array was scanned using the iScan (Illumina)
|
| Description |
Genomic DNA extracted from cells was genotyped using HumanCytoSNP-12 v2.1 DNA Analysis BeadChip Kit (Illumina).
|
| Data processing |
Data were processed using GenomeStudio V2011.1
|
| |
|
| Submission date |
Jan 30, 2014 |
| Last update date |
May 20, 2015 |
| Contact name |
Akihiro Umezawa |
| E-mail(s) |
umezawa@1985.jukuin.keio.ac.jp
|
| Phone |
81-3-5494-7047
|
| Organization name |
National Center for Child Health and Development
|
| Department |
Center for Regenerative Medicine
|
| Street address |
2-10-1 Okura
|
| City |
Setagaya-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
157-8535 |
| Country |
Japan |
| |
|
| Platform ID |
GPL13829 |
| Series (1) |
| GSE54576 |
An analysis of genomic structural alteration in three human ES cell lines. |
|
