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| Status |
Public on May 22, 2007 |
| Title |
Glis3: The role in osteoblast differentiation and adipocyte differentiation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The expression of Glis3 in C3H10T1/2 cells promotes osteoblastic differentiation as indicated by the the induction of increase in alkaline phosphatase activity, an early marker of osteoblast differentiation, and increased expression of osteopontin, a late marker of osteogenesis. Glis3 acts synergistically with bone morphogenic protein 2 (BMP-2). In contrast, expression of Glis3 inhibits the induction of adipocyte differentiation. Microarray analysis identified the fibroblast growth factor 18 (FGF18) as one of the genes induced by Glis3 in C3H10T1/2 cells directly.
Keywords: Glis3, osteoblast differentiation, adipocyte differentiation, FGF18, BMP2
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| Overall design |
Total RNA was extracted from C3H10T1/2 cells infected with empty vector or Glis3 using Qiagen Rneasy Mini kit. Each RNA sample was amplified and converted to fluorescently labeled cRNA, either with cyanine 3 (Cy3) or cyanine 5 (Cy5), using the Agilent Fluorescent Linear Amplification Kit. The fluorescently labeled cRNAs were mixed and hybridized simultaneously to Agilent mouse oligo arrays. The sample pair was hybridized to two arrays, employing a fluor reversal. Chips were scanned with an Agilent dual-laser scanner. Gene expression data were generated using the Agilent feature extraction software (v7.5), with defaults for all parameters.
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| Contributor(s) |
Beak J, Kang H, Kim Y, Jetten AM |
| Citation(s) |
17488195 |
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| Submission date |
Jul 24, 2006 |
| Last update date |
Dec 06, 2012 |
| Contact name |
NIEHS Microarray Core |
| E-mail(s) |
microarray@niehs.nih.gov, liuliw@niehs.nih.gov
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| Organization name |
NIEHS
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| Department |
DIR
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| Lab |
Microarray Core
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| Street address |
111 T.W. Alexander Drive
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| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
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| Platforms (1) |
| GPL891 |
Agilent-011978 Mouse Microarray G4121A (Feature Number version) |
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| Samples (2) |
| GSM122771 |
10T 1/2 Con vs. 10T 1/2 Glis3 (ExpID 2922) |
| GSM122772 |
10T 1/2 Glis3 vs. 10T 1/2 Con (ExpID 2922) |
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| Relations |
| BioProject |
PRJNA96285 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE5379_RAW.tar |
61.7 Mb |
(http)(custom) |
TAR (of TIFF, TXT) |
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