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Series GSE52810 Query DataSets for GSE52810
Status Public on Nov 28, 2013
Title The effect of miR196a of overall gene expression in oral keratinocytes
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We wished to assess the effect of miR196a on overall gene expression in reducing it in high expressing epithelial cells (D19 and B16) using antimiR196a and over expressing it in low-expressing cells (OKF4) using pre-miR196a. We transfected anti miR196a in to D19 and B16 oral keratinocytes which express high levels of miR196a in triplicate cultures, harvesting 24h after transfection. We transfected pre-miR196 in okf4 immorualised normal oral keratinocytes in duplicate culture. Successful transfection was ensured by qPCR.
Overall design 16 Samples in total. 2 high expressing cells: control and anti-miR transfected, in triplicate. 1 low expressing cell: control and premiR transfected, in duplicate
Contributor(s) Hunter KD, Lambert DW
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Submission date Nov 27, 2013
Last update date Jan 09, 2018
Contact name Keith David Hunter
Organization name University of Sheffield
Department School of Clinical Dentistry
Lab Oral Pathology
Street address Claremont Crescent
City Sheffield
State/province South Yorkshire
ZIP/Postal code S26 7YJ
Country United Kingdom
Platforms (1)
GPL17077 Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version)
Samples (16)
GSM1276569 B16_control_rep1
GSM1276570 B16_control_rep2
GSM1276571 B16_control_rep3
BioProject PRJNA230116

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE52810_RAW.tar 50.0 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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