|
| Status |
Public on Nov 28, 2013 |
| Title |
The effect of miR196a of overall gene expression in oral keratinocytes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
We wished to assess the effect of miR196a on overall gene expression in reducing it in high expressing epithelial cells (D19 and B16) using antimiR196a and over expressing it in low-expressing cells (OKF4) using pre-miR196a. We transfected anti miR196a in to D19 and B16 oral keratinocytes which express high levels of miR196a in triplicate cultures, harvesting 24h after transfection. We transfected pre-miR196 in okf4 immorualised normal oral keratinocytes in duplicate culture. Successful transfection was ensured by qPCR.
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| |
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| Overall design |
16 Samples in total. 2 high expressing cells: control and anti-miR transfected, in triplicate. 1 low expressing cell: control and premiR transfected, in duplicate
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| |
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| Contributor(s) |
Hunter KD, Lambert DW |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Nov 27, 2013 |
| Last update date |
Jan 09, 2018 |
| Contact name |
Keith David Hunter |
| E-mail(s) |
k.hunter@sheffield.ac.uk
|
| Organization name |
University of Sheffield
|
| Department |
School of Clinical Dentistry
|
| Lab |
Oral Pathology
|
| Street address |
Claremont Crescent
|
| City |
Sheffield |
| State/province |
South Yorkshire |
| ZIP/Postal code |
S26 7YJ |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL17077 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version) |
|
| Samples (16)
|
|
| Relations |
| BioProject |
PRJNA230116 |
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