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Series GSE52048 Query DataSets for GSE52048
Status Public on Nov 03, 2016
Title Palmitate-induced inhibition of C2C12 insulin pathway modifies the release of exosomes and their biological actions on muscle cells
Organism Mus musculus
Experiment type Expression profiling by array
Summary Exosomes are cell-released bioactive nanovesicles now considered as new partners in the inter-organ cross-talks. The possibility that skeletal muscle (SkM)-derived exosomes act as a mode of systemic communication has hitherto never been described. Thus, in this study we have tested the hypothesis that through the exosomal route, muscle cells might transmit specific signals during insulin-resistance.
Overall design C2C12 myoblasts were differentiated into myotubes and then treated with 0.5mM palmitate in order to alter insulin signaling pathway or BSA as control. Exosomes released from palmitate-treated cells (EXO-palm) and from BSA-treated cells (EXO-BSA) were collected by ultracentrifugation. Then C2C12 were treated with either EXO-palm or EXO-BSA. RNA was extracted and global transcriptomic analysis was performed.
Contributor(s) Rome S, Meugnier E
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Submission date Nov 04, 2013
Last update date Jan 19, 2018
Contact name emmanuelle meugnier
Organization name INSERMU1060/INRA1397
Lab CarMeN
Street address Cens Eli Bat 2 D- Hopital Lyon Sud
City Pierre BĂ©nite
ZIP/Postal code 69495
Country France
Platforms (1)
GPL10333 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Feature Number version)
Samples (7)
GSM1257984 BSA3
GSM1257985 BSA4
GSM1257986 BSA5
BioProject PRJNA226583

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Supplementary file Size Download File type/resource
GSE52048_RAW.tar 63.5 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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