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Sample GSM1257984 Query DataSets for GSM1257984
Status Public on Nov 03, 2016
Title BSA3
Sample type RNA
 
Source name C2C12 incubated with Exo-BSA
Organism Mus musculus
Characteristics cell line: C2C12
cell type: myoblast
Extracted molecule total RNA
Extraction protocol RNA was prepared using Trizol (Life Technologies) following the manufacturer's recommendations. RNA was quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).
Label Cyanine3
Label protocol Cyanine-3 (Cy3) labeled cRNA was prepared from 0.1 ug RNA using the One-Color Low RNA Input Linear Amplification PLUS kit (Agilent) according to the manufacturer's instructions, followed by RNAeasy column purification (QIAGEN). Dye incorporation and cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer.
 
Hybridization protocol 1.65 ug of Cy3-labelled cRNA (specific activity >10.0 pmol Cy3/ug cRNA) was fragmented at 60°C for 30 minutes in a reaction volume of 55µl containing 1x Agilent fragmentation buffer following the manufacturers instructions. On completion of the fragmentation reaction,55µl of 2x Agilent hybridization buffer was added to the fragmentation mixture and 100µl were hybridized to Agilent Whole Mouse Genome Oligo Microarrays (G4112A) for 17 hours at 65°C in a rotating Agilent hybridization oven. After hybridization, microarrays were washed according to manufacturer's intructions
Scan protocol Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505B) using one color scan setting for 4x44k array slides
Description control, biological replicate 1
Data processing The scanned images were analyzed with Feature Extraction Software 9.1 (Agilent) using default parameters to obtain background subtracted . Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded. Raw data were normalized using bioconductor and limma package. Briefly, background substraction was performed using the normexp correction with an offset=1, then signals were normalized using quantile normalization. Only spots presenting a signal 10% brighter than the 95th percentile of the negative control are kept for statistical analysis
 
Submission date Nov 04, 2013
Last update date Nov 03, 2016
Contact name emmanuelle meugnier
E-mail(s) meugnier@univ-lyon1.fr
Organization name INSERMU1060/INRA1397
Lab CarMeN
Street address Cens Eli Bat 2 D- Hopital Lyon Sud
City Pierre Bénite
ZIP/Postal code 69495
Country France
 
Platform ID GPL10333
Series (1)
GSE52048 Palmitate-induced inhibition of C2C12 insulin pathway modifies the release of exosomes and their biological actions on muscle cells

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
1 13.1097432930036
2 3.76434667769684
3 4.09687960660143
4 4.35378563839028
5 4.50409896137349
6 4.40600057880525
7 4.49509966882632
8 4.260111176752
9 4.32008015584249
10 4.25504780701257
11 3.69523079416523
12 7.29180431067237
13 5.25160104436155
14 10.6893946453317
15 6.35045094526785
16 11.0466759972259
17 13.8632259683155
18 5.1162538137344
19 5.3569885308097
20 15.1119184803323

Total number of rows: 44397

Table truncated, full table size 981 Kbytes.




Supplementary file Size Download File type/resource
GSM1257984_US45103047_252665511862_S01_GE1_107_Sep09_1_1.txt.gz 9.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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